WB (Western Blot)
(Western blot analysis of POLR2D (arrow) using rabbit polyclonal POLR2D Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR2D gene.)
Rabbit POLR2D Polyclonal Antibody | anti-POLR2D antibody
POLR2D Antibody (Center)
Gene Names
POLR2D; RBP4; RPB16; HSRBP4; HSRPB4
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
POLR2D, Antibody; POLR2D Antibody (Center); DNA-directed RNA polymerase II subunit RPB4; RNA polymerase II subunit B4; DNA-directed RNA polymerase II subunit D; RNA polymerase II 16 kDa subunit; RPB16; POLR2D; anti-POLR2D antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This POLR2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the Central region of human POLR2D.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
51-79
Sequence Length
142
Applicable Applications for anti-POLR2D antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of POLR2D (arrow) using rabbit polyclonal POLR2D Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR2D gene.)
WB (Western Blot)
(Western blot analysis of POLR2D (arrow) using rabbit polyclonal POLR2D Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR2D gene.)
WB (Western Blot)
(POLR2D Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the POLR2D antibody detected the POLR2D protein (arrow).)
WB (Western Blot)
(POLR2D Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the POLR2D antibody detected the POLR2D protein (arrow).)
Related Product Information for anti-POLR2D antibody
This gene encodes the fourth largest subunit of RNA
polymerase II, the polymerase responsible for synthesizing
messenger RNA in eukaryotes. In yeast, this polymerase subunit is
associated with the polymerase under suboptimal growth conditions
and may have a stress protective role. A sequence for a ribosomal
pseudogene is contained within the 3' untranslated region of the
transcript from this gene.
polymerase II, the polymerase responsible for synthesizing
messenger RNA in eukaryotes. In yeast, this polymerase subunit is
associated with the polymerase under suboptimal growth conditions
and may have a stress protective role. A sequence for a ribosomal
pseudogene is contained within the 3' untranslated region of the
transcript from this gene.
References
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Meka, H., et al. Nucleic Acids Res. 33(19):6435-6444(2005)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)
Kaehlcke, K., et al. Mol. Cell 12(1):167-176(2003)
Shilatifard, A., et al. Annu. Rev. Biochem. 72, 693-715 (2003) :
Meka, H., et al. Nucleic Acids Res. 33(19):6435-6444(2005)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)
Kaehlcke, K., et al. Mol. Cell 12(1):167-176(2003)
Shilatifard, A., et al. Annu. Rev. Biochem. 72, 693-715 (2003) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16311
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB4
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide D
NCBI Official Symbol
POLR2D
NCBI Official Synonym Symbols
RBP4; RPB16; HSRBP4; HSRPB4
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB4
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB4
UniProt Gene Name
POLR2D
UniProt Synonym Gene Names
RNA polymerase II subunit B4; RPB16
UniProt Entry Name
RPB4_HUMAN
Similar Products
Product Notes
The POLR2D polr2d (Catalog #AAA287449) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 51-79. The POLR2D Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's POLR2D can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the POLR2D polr2d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR2D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.