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IF (Immunofluorescence)
(Figure 4 Immunofluorescence Validation of POLRMT in Rat BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling POLRMT with AAA40966 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Rabbit POLRMT Polyclonal Antibody | anti-POLRMT antibody
POLRMT (CT) Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, ELISA
Purity
POLRMT Antibody is affinity chromatography purified via peptide column.
Synonyms
POLRMT, Antibody; POLRMT (CT) Antibody; DNA-directed RNA polymerase; mitochondrial; MtRPOL; POLRMT Isoforms; RNA polymerase mitochondrial; anti-POLRMT antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
POLRMT Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; POLRMT Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-POLRMT antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen
Anti-POLRMT antibody (9561) was raised against a peptide corresponding to 11 amino acids near the carboxyl terminus of human POLRMT.
The immunogen is located within the last 50 amino acids of POLRMT.
The immunogen is located within the last 50 amino acids of POLRMT.
Homology
Predicted species reactivity based on immunogen sequence: Cow (82%); Chimpanzee (100%) ; monkey (82%); Cat (90%)
Research Area
Signal Transduction
NCBI Organism
Homo sapiens
Isoforms
Human POLRMT has 1 isoform (1230aa, 138.6kD). Mouse POLRMT has one isoform (1207aa, 136.7kD). Rat POLRMT has one isoform (1203aa, 135.8kD). can detect POLRMT in human, mouse and rat.
Preparation and Storage
POLRMT antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Figure 4 Immunofluorescence Validation of POLRMT in Rat BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling POLRMT with AAA40966 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 4 Immunofluorescence Validation of POLRMT in Rat BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling POLRMT with AAA40966 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 3 Immunofluorescence Validation of POLRMT in Mouse BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling POLRMT with AAA40966 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 3 Immunofluorescence Validation of POLRMT in Mouse BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling POLRMT with AAA40966 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 2 Immunofluorescence Validation of POLRMT in Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling POLRMT with AAA40966 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 2 Immunofluorescence Validation of POLRMT in Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling POLRMT with AAA40966 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Figure 1 WB Validation in Human Cell Lines Loading: 15ug of lysate. Antibodies: POLRMT, 2ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1 WB Validation in Human Cell Lines Loading: 15ug of lysate. Antibodies: POLRMT, 2ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
Related Product Information for anti-POLRMT antibody
POLRMT Antibody: This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication. POLRMT mutations impair mitochondrial transcription causing neurological disease. Knock-down of MRPL12 by RNA interference results in instability of POLRMT.
Product Categories/Family for anti-POLRMT antibody
References
Inatomi et al. Biochim Biophys Acta Mol Cell Res. 2022; 1869(1); 119167
Olahova et al. Nat Commun. 2021; 12(1):1135
Nouws et al. J Biol Chem 2016; 291(2):989-997
Olahova et al. Nat Commun. 2021; 12(1):1135
Nouws et al. J Biol Chem 2016; 291(2):989-997
NCBI and Uniprot Product Information
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Product Notes
The POLRMT (Catalog #AAA40966) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLRMT (CT) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POLRMT can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the POLRMT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLRMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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