FCM/FACS (Flow Cytometry)
(PPARA Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit PPARA Polyclonal Antibody | anti-PPARA antibody
PPARA Antibody (Center)
Gene Names
PPARA; PPAR; NR1C1; hPPAR; PPARalpha
Reactivity
Mouse (Predicted Reactivity: Rat, Xenopus)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PPARA, Antibody; PPARA Antibody (Center); Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1; PPARA; NR1C1; PPAR; anti-PPARA antibody
Host
Rabbit
Reactivity
Mouse (Predicted Reactivity: Rat, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PPARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-181 amino acids from the Central region of human PPARA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
153-181
Sequence Length
468
Applicable Applications for anti-PPARA antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PPARA Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PPARA Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(PPARA Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PPARA antibody detected the PPARA protein (arrow).)
WB (Western Blot)
(PPARA Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PPARA antibody detected the PPARA protein (arrow).)
Related Product Information for anti-PPARA antibody
Peroxisome proliferators include hypolipidemic drugs,
herbicides, leukotriene antagonists, and plasticizers; this term
arises because they induce an increase in the size and number of
peroxisomes. Peroxisomes are subcellular organelles found in plants
and animals that contain enzymes for respiration and for
cholesterol and lipid metabolism. The action of peroxisome
proliferators is thought to be mediated via specific receptors,
called PPARs, which belong to the steroid hormone receptor
superfamily. PPARs affect the expression of target genes involved
in cell proliferation, cell differentiation and in immune and
inflammation responses. Three closely related subtypes (alpha,
beta/delta, and gamma) have been identified. This gene encodes the
subtype PPAR-alpha, which is a nuclear transcription factor.
Multiple alternatively spliced transcript variants have been
described for this gene, although the full-length nature of only
two has been determined.
herbicides, leukotriene antagonists, and plasticizers; this term
arises because they induce an increase in the size and number of
peroxisomes. Peroxisomes are subcellular organelles found in plants
and animals that contain enzymes for respiration and for
cholesterol and lipid metabolism. The action of peroxisome
proliferators is thought to be mediated via specific receptors,
called PPARs, which belong to the steroid hormone receptor
superfamily. PPARs affect the expression of target genes involved
in cell proliferation, cell differentiation and in immune and
inflammation responses. Three closely related subtypes (alpha,
beta/delta, and gamma) have been identified. This gene encodes the
subtype PPAR-alpha, which is a nuclear transcription factor.
Multiple alternatively spliced transcript variants have been
described for this gene, although the full-length nature of only
two has been determined.
Product Categories/Family for anti-PPARA antibody
References
Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)
Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Eynon, N., et al. Mitochondrion (2010) In press :
Aldhoon, B., et al. Folia Biol. (Praha) 56(3):116-123(2010)
Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Eynon, N., et al. Mitochondrion (2010) In press :
Aldhoon, B., et al. Folia Biol. (Praha) 56(3):116-123(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52225
NCBI Official Full Name
peroxisome proliferator-activated receptor alpha
NCBI Official Synonym Full Names
peroxisome proliferator-activated receptor alpha
NCBI Official Symbol
PPARA
NCBI Official Synonym Symbols
PPAR; NR1C1; hPPAR; PPARalpha
NCBI Protein Information
peroxisome proliferator-activated receptor alpha
UniProt Protein Name
Peroxisome proliferator-activated receptor alpha
UniProt Gene Name
PPARA
UniProt Synonym Gene Names
NR1C1; PPAR; PPAR-alpha
UniProt Entry Name
PPARA_HUMAN
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Product Notes
The PPARA ppara (Catalog #AAA289234) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 153-181. The PPARA Antibody (Center) reacts with Mouse (Predicted Reactivity: Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's PPARA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PPARA ppara for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPARA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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