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IF (Immunofluorescence)
(Immunofluorescence of PPARGC1A in rat heart tissue with PPARGC1A antibody at 20 μg/mL.)
Rabbit PPARGC1A Polyclonal Antibody | anti-PPARGC1A antibody
PPARGC1A Antibody
Gene Names
PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PPARGC1A antibody is affinity chromatography purified via peptide column.
Synonyms
PPARGC1A; Polyclonal Antibody; PPARGC1A Antibody; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha); Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Ligand effect modulator 6; PGC-1-alpha; peroxisome proliferator-activated receptor gamma; coactivator 1 alpha; anti-PPARGC1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PPARGC1A antibody is human, mouse and rat reactive. At least three isoforms of PPARGC1A are known to exist; this antibody will detect all three isoforms.
Purity/Purification
PPARGC1A antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; PPARGC1A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
798
Applicable Applications for anti-PPARGC1A antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-paraffin (IHC-p), Immunofluroescence (IF)
Application Notes
Optimal dilutions for each application to be determined by the researcher.
Conjugate
Unconjugated
Immunogen
PPARGC1A antibody was raised against a 17 amino acid peptide near the amino terminus of human PPARGC1A.
The immunogen is located within amino acids 200 - 250 of PPARGC1A.
The immunogen is located within amino acids 200 - 250 of PPARGC1A.
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (94%)
Preparation and Storage
PPARGC1A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
IF (Immunofluorescence)
(Immunofluorescence of PPARGC1A in rat heart tissue with PPARGC1A antibody at 20 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of PPARGC1A in rat heart tissue with PPARGC1A antibody at 20 μg/mL.)
IHC (Immunohistochemistry)
(Immunohistochemistry of PPARGC1A in rat heart tissue with PPARGC1A antibody at 5 μg/mL.)
IHC (Immunohistochemistry)
(Immunohistochemistry of PPARGC1A in rat heart tissue with PPARGC1A antibody at 5 μg/mL.)
IHC (Immunohistochemistry)
(Immunohistochemistry of PPARGC1A in rat heart tissue with PPARGC1A antibody at 5 μg/mL.)
IHC (Immunohistochemistry)
(Immunohistochemistry of PPARGC1A in rat heart tissue with PPARGC1A antibody at 5 μg/mL.)
WB (Western Blot)
(WB Validation in Rat Kidney Loading: 15 ?g of rat kidney lysate Antibodies: PPARGC1A 7793, 2 ?g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(WB Validation in Rat Kidney Loading: 15 ?g of rat kidney lysate Antibodies: PPARGC1A 7793, 2 ?g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(WB Validation in Mouse Skin Loading: 15 ?g of mouse skin lysate Antibodies: PPARGC1A 7793, 2 ?g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(WB Validation in Mouse Skin Loading: 15 ?g of mouse skin lysate Antibodies: PPARGC1A 7793, 2 ?g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(WB Validation in Human Lung Loading: 15 ?g of human lung lysate Antibodies: PPARGC1A 7793, 2 ?g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(WB Validation in Human Lung Loading: 15 ?g of human lung lysate Antibodies: PPARGC1A 7793, 2 ?g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(WB Validation in Human Cell Lines Loading: 15 ?g of human cell lysate Antibodies: PPARGC1A 7793, 2 ?g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(WB Validation in Human Cell Lines Loading: 15 ?g of human cell lysate Antibodies: PPARGC1A 7793, 2 ?g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Western blot analysis of PPARGC1A in Human Skeletal Muscle tissue lysate with PPARGC1A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
WB (Western Blot)
(Western blot analysis of PPARGC1A in Human Skeletal Muscle tissue lysate with PPARGC1A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
WB (Western Blot)
(Western blot analysis of PPARGC1A in human heart tissue lysate with PPARGC1A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
WB (Western Blot)
(Western blot analysis of PPARGC1A in human heart tissue lysate with PPARGC1A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Related Product Information for anti-PPARGC1A antibody
The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), also known as LEM-6, is a transcriptional coactivator that regulates the genes involved in energy metabolism (1). PPARGC1A interacts with PPARgamma, which permits the interaction of PPARGC1A with multiple transcription factors. PPARGC1A can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination (2). PPARGC1A may be also involved in the development of obesity (3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 91kD
Observed: 100 kD
Observed: 100 kD
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
NCBI Official Synonym Full Names
peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
NCBI Official Symbol
PPARGC1A
NCBI Official Synonym Symbols
LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)
NCBI Protein Information
peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; L-PGC-1alpha; PPARGC-1-alpha; ligand effect modulator-6; PPAR gamma coactivator variant form; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Gene Name
PPARGC1A
UniProt Synonym Gene Names
LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha
UniProt Entry Name
PRGC1_HUMAN
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Product Notes
The PPARGC1A ppargc1a (Catalog #AAA10967) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPARGC1A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPARGC1A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-paraffin (IHC-p), Immunofluroescence (IF). Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the PPARGC1A ppargc1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPARGC1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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