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WB (Western Blot)
(Western blot analysis of PPID (arrow) using rabbit polyclonal PPID Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPID gene.)
Rabbit PPID Polyclonal Antibody | anti-PPID antibody
PPID Antibody (N-term)
Gene Names
PPID; CYPD; CYP-40
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Western Blot, Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PPID, Antibody; PPID Antibody (N-term); Peptidyl-prolyl cis-trans isomerase D; PPIase D; 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin-40; CYP-40; Cyclophilin-related protein; Rotamase D; PPID; CYP40; CYPD; anti-PPID antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PPID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-115 amino acids from the N-terminal region of human PPID.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
85-115
Sequence Length
370
Applicable Applications for anti-PPID antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of PPID (arrow) using rabbit polyclonal PPID Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPID gene.)
WB (Western Blot)
(Western blot analysis of PPID (arrow) using rabbit polyclonal PPID Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPID gene.)
FCM/FACS (Flow Cytometry)
(PPID Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PPID Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PPID Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PPID Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of PPID Antibody (N-term) in NIH-3T3 cell line lysates (35ug/lane). PPID (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PPID Antibody (N-term) in NIH-3T3 cell line lysates (35ug/lane). PPID (arrow) was detected using the purified Pab.)
Related Product Information for anti-PPID antibody
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Product Categories/Family for anti-PPID antibody
References
Mayya V.,et.al., Sci. Signal. 2:RA46-RA46(2009).
Gevaert K., et.al., Nat. Biotechnol. 21:566-569(2003).
Gevaert K., et.al., Nat. Biotechnol. 21:566-569(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40764
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase D
NCBI Official Synonym Full Names
peptidylprolyl isomerase D
NCBI Official Symbol
PPID
NCBI Official Synonym Symbols
CYPD; CYP-40
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase D
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase D
UniProt Gene Name
PPID
UniProt Synonym Gene Names
CYP40; CYPD; PPIase D; CYP-40
UniProt Entry Name
PPID_HUMAN
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Product Notes
The PPID ppid (Catalog #AAA285653) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 85-115. The PPID Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PPID can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PPID ppid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPID, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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