WB (Western Blot)
(Detection of mouse PPIP5K2 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPIP5K2 antibody (AAA212527 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse PPIP5K2 Polyclonal Antibody | anti-PPIP5K2 antibody
Rabbit anti-PPIP5K2 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PPIP5K2, Antibody; Rabbit anti-PPIP5K2 Antibody, Affinity Purified; IP7K2; VIP1 homolog 2; insP6 and PP-IP5 kinase 2; inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2; inositol heptaphosphate kinase 2; hsVIP2; HISPPD1; Diphosphoinositol pentakisphosphate kinase 2; DFNB100; deafness, autosomal recessive 100; CFAP160; VIP2; histidine acid phosphatase domain-containing protein 1; Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2; diphosphoinositol pentakisphosphate kinase 2; anti-PPIP5K2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PPIP5K2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 925 and 975
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse PPIP5K2 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPIP5K2 antibody (AAA212527 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse PPIP5K2 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPIP5K2 antibody (AAA212527 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PPIP5K2 by western blot. Samples: Whole cell lysate (10 ug) from K-562, GaMG, A-549, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPIP5K2 antibody (AAA212527 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PPIP5K2 by western blot. Samples: Whole cell lysate (10 ug) from K-562, GaMG, A-549, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPIP5K2 antibody (AAA212527 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PPIP5K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPIP5K2 antibody (AAA212527 lot 2) used for IP at 6 ug per reaction. PPIP5K2 was also immunoprecipitated by a previous lot of this antibody (AAA212527 lot 1) and another rabbit anti-PPIP5K2 antibody . For blotting immunoprecipitated PPIP5K2, AAA212527 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human PPIP5K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPIP5K2 antibody (AAA212527 lot 2) used for IP at 6 ug per reaction. PPIP5K2 was also immunoprecipitated by a previous lot of this antibody (AAA212527 lot 1) and another rabbit anti-PPIP5K2 antibody . For blotting immunoprecipitated PPIP5K2, AAA212527 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-PPIP5K2 antibody
PPIP5K2 is a bifunctional inositol kinase that acts in concert with the IP6K kinases IP6K1, IP6K2 and IP6K3 to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, exocytosis, insulin signaling and neutrophil activation. PPPIP5K2 phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, PPIP5K2 phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43314].
Product Categories/Family for anti-PPIP5K2 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PPIP5K2 ppip5k2 (Catalog #AAA212527) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PPIP5K2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPIP5K2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPIP5K2 ppip5k2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPIP5K2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.