WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using PPM1L antibody.)
Rabbit PPM1L Polyclonal Antibody | anti-PPM1L antibody
PPM1L Antibody
Gene Names
PPM1L; PP2CE; PPM1-LIKE; PP2C-epsilon
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PPM1L, Antibody; PPM1L Antibody; EC 3.1.3.16; in phosphatase 1 (formerly 2C) like; MGC132545; MGC132547; OTTHUMP00000213920; OTTHUMP00000213921; PP2C epsilon; PP2C-epsilon; PP2CE; PPM1 like; PPM1-LIKE; PPM1L; PPM1L_HUMAN; Protein phosphatase 1 (formerly 2C)-like; Protein phosphatase 1 like; Protein phosphatase 1-like; Protein phosphatase 1L; Protein phosphatase 2C epsilon isoform; Protein phosphatase 2C isoform epsilon; Protein phosphatase, Mg2+/Mn2+ dependent, 1L; anti-PPM1L antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PPM1L antibody detects endogenous levels of total PPM1L
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
233
Applicable Applications for anti-PPM1L antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Membrane.
Tissue Specificity
Ubiquitous. Highly expressed in heart, placenta, lung, liver, kidney and pancreas.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using PPM1L antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using PPM1L antibody.)
IF (Immunofluorescence)
(AAA325005 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA325005 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-PPM1L antibody
Function: Acts as a suppressor of the SAPK signaling pathways by associating with and dephosphorylating MAP3K7/TAK1 and MAP3K5, and by attenuating the association between MAP3K7/TAK1 and MAP2K4 or MAP2K6.
Subunit Structure: Interacts with MAP3K7/TAK1 (By similarity). Interacts with MAP3K5.
Similarity: Belongs to the PP2C family.
Subunit Structure: Interacts with MAP3K7/TAK1 (By similarity). Interacts with MAP3K5.
Similarity: Belongs to the PP2C family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 48 kD
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
protein phosphatase 1L isoform 2
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1L
NCBI Official Symbol
PPM1L
NCBI Official Synonym Symbols
PP2CE; PPM1-LIKE; PP2C-epsilon
NCBI Protein Information
protein phosphatase 1L
UniProt Protein Name
Protein phosphatase 1L
UniProt Gene Name
PPM1L
UniProt Synonym Gene Names
PP2CE; PP2C-epsilon
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Product Notes
The PPM1L ppm1l (Catalog #AAA325005) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPM1L Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1L can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the PPM1L ppm1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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