IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using PPP1R12A antibody at dilution of1:100 (x40 lens).)
Rabbit PPP1R12A Polyclonal Antibody | anti-PPP1R12A antibody
PPP1R12A Polyclonal Antibody
Gene Names
PPP1R12A; MBS; M130; MYPT1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
PPP1R12A , Antibody; PPP1R12A Polyclonal Antibody; MBS; M130; MYPT1; anti-PPP1R12A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Applicable Applications for anti-PPP1R12A antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant protein of human PPP1R12A
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-17434 / sc-17433 / sc-34142 / sc-34143 / sc-25618
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using PPP1R12A antibody at dilution of1:100 (x40 lens).)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using PPP1R12A antibody at dilution of1:100 (x40 lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PPP1R12A antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PPP1R12A antibody.)
Related Product Information for anti-PPP1R12A antibody
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 115kDa
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 12A isoform d
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory subunit 12A
NCBI Official Symbol
PPP1R12A
NCBI Official Synonym Symbols
MBS; M130; MYPT1
NCBI Protein Information
protein phosphatase 1 regulatory subunit 12A
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 12A
UniProt Gene Name
PPP1R12A
UniProt Synonym Gene Names
MBS; MYPT1; Myosin phosphatase target subunit 1
UniProt Entry Name
MYPT1_HUMAN
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Product Notes
The PPP1R12A ppp1r12a (Catalog #AAA172548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP1R12A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1R12A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PPP1R12A ppp1r12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP1R12A , Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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