IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#AAA330061) and mouse anti-beta tubulin Ab(# ) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
Rabbit anti-Human, Mouse PPP1R12C Polyclonal Antibody | anti-PPP1R12C antibody
PPP1R12C Antibody
Gene Names
PPP1R12C; p84; p85; LENG3; MBS85
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
PPP1R12C, Antibody; PPP1R12C Antibody; LENG 3; LENG3; leukocyte receptor cluster (LRC) encoded novel gene 3; leukocyte receptor cluster (LRC) member 3; MBS 85; MBS85; Myosin binding subunit 85; p84; p85; PP12C_HUMAN; Ppp1r12c; protein phosphatase 1 regulatory (inhibitor) subunit 12C; protein phosphatase 1 myosin binding subunit of 85 kDa; protein phosphatase 1 myosin binding subunit p85; Protein phosphatase 1 myosin-binding subunit of 85 kDa; Protein phosphatase 1 myosin-binding subunit p85; Protein phosphatase 1 regulatory subunit 12C; anti-PPP1R12C antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-PPP1R12C antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human PPP1R12C.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#AAA330061) and mouse anti-beta tubulin Ab(# ) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#AAA330061) and mouse anti-beta tubulin Ab(# ) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
WB (Western Blot)
(Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using PPP1R12C Ab at 1/1000 dilution. Observed bands:70kD.)
WB (Western Blot)
(Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using PPP1R12C Ab at 1/1000 dilution. Observed bands:70kD.)
Product Categories/Family for anti-PPP1R12C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 12C isoform b
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 12C
NCBI Official Symbol
PPP1R12C
NCBI Official Synonym Symbols
p84; p85; LENG3; MBS85
NCBI Protein Information
protein phosphatase 1 regulatory subunit 12C; myosin-binding subunit 85; leukocyte receptor cluster (LRC) member 3; protein phosphatase 1 myosin-binding subunit p85; leukocyte receptor cluster (LRC) encoded novel gene 3; protein phosphatase 1 myosin-bindi
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 12C
UniProt Gene Name
PPP1R12C
UniProt Synonym Gene Names
LENG3; MBS85; Protein phosphatase 1 myosin-binding subunit p85
UniProt Entry Name
PP12C_HUMAN
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Product Notes
The PPP1R12C ppp1r12c (Catalog #AAA330061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP1R12C Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1R12C can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the PPP1R12C ppp1r12c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP1R12C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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