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WB (Western Blot)
(PPP2R2A Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PPP2R2A antibody detected the PPP2R2A protein (arrow).)
Rabbit PPP2R2A Polyclonal Antibody | anti-PPP2R2A antibody
PPP2R2A Antibody (N-term)
Gene Names
PPP2R2A; B55A; PR52A; PR55A; B55ALPHA
Reactivity
Human (Predicted Reactivity: Monkey, Mouse, Pig, Rabbit, Rat)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PPP2R2A, Antibody; PPP2R2A Antibody (N-term); Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform; PP2A subunit B isoform B55-alpha; PP2A subunit B isoform PR55-alpha; PP2A subunit B isoform R2-alpha; PP2A subunit B isoform alpha; PPP2R2A; anti-PPP2R2A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey, Mouse, Pig, Rabbit, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PPP2R2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human PPP2R2A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
43-71
Sequence Length
457
Applicable Applications for anti-PPP2R2A antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(PPP2R2A Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PPP2R2A antibody detected the PPP2R2A protein (arrow).)
WB (Western Blot)
(PPP2R2A Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PPP2R2A antibody detected the PPP2R2A protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with PPP2R2A Antibody (N-term). AAA290202 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with PPP2R2A Antibody (N-term). AAA290202 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using PPP2R2A Antibody (N-term). AAA290202 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using PPP2R2A Antibody (N-term). AAA290202 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. liver section using PPP2R2A Antibody (N-term). AAA290202 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. liver section using PPP2R2A Antibody (N-term). AAA290202 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Related Product Information for anti-PPP2R2A antibody
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Product Categories/Family for anti-PPP2R2A antibody
References
Mayer R.E., et al. Biochemistry 30:3589-3597(1991).
Ota T., et al. Nat. Genet. 36:40-45(2004).
Nusbaum C., et al. Nature 439:331-335(2006).
Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Li H.H., et al. EMBO J. 26:402-411(2007).
Ota T., et al. Nat. Genet. 36:40-45(2004).
Nusbaum C., et al. Nature 439:331-335(2006).
Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Li H.H., et al. EMBO J. 26:402-411(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51692
NCBI Official Full Name
serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform isoform 2
NCBI Official Synonym Full Names
protein phosphatase 2, regulatory subunit B, alpha
NCBI Official Symbol
PPP2R2A
NCBI Official Synonym Symbols
B55A; PR52A; PR55A; B55ALPHA
NCBI Protein Information
serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
UniProt Gene Name
PPP2R2A
UniProt Entry Name
2ABA_HUMAN
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Product Notes
The PPP2R2A ppp2r2a (Catalog #AAA290202) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 43-71. The PPP2R2A Antibody (N-term) reacts with Human (Predicted Reactivity: Monkey, Mouse, Pig, Rabbit, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PPP2R2A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PPP2R2A ppp2r2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP2R2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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