WB (Western Blot)
(Detection of human and mouse PPP2R2B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP2R2B antibody AAA211252 (lot AAA211252-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse PPP2R2B Polyclonal Antibody | anti-PPP2R2B antibody
Rabbit anti-PPP2R2B Antibody, Affinity Purified
Gene Names
PPP2R2B; PR52B; SCA12; B55BETA; PR55BETA; PP2ABBETA; PP2APR55B; PR2ABBETA; PR55-BETA; PP2AB55BETA; PR2AB55BETA; PP2APR55BETA; PR2APR55BETA
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PPP2R2B, Antibody; Rabbit anti-PPP2R2B Antibody, Affinity Purified; serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform; PR2APR55BETA; PR55BETA; PR55-BETA; protein phosphatase 2, regulatory subunit B, beta; SCA12; PR2AB55BETA; serine/threonine protein phosphatase 2A, neuronal isoform; PR2ABBETA; serine/threonine protein phosphatase 2A, 55 kDa regulatory subunit B, beta isoform; PP2A subunit B isoform beta; PR52B; PP2A subunit B isoform B55-beta; PP2APR55BETA; PP2A subunit B isoform PR55-beta; PP2A subunit B isoform R2-beta; PP2A, subunit B, B-beta isoform; PP2AB55BETA; PP2ABBETA; PP2APR55B; B55BETA; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform; protein phosphatase 2 regulatory subunit Bbeta; anti-PPP2R2B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PPP2R2B antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Rabbit, Pig, Orangutan
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse PPP2R2B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP2R2B antibody AAA211252 (lot AAA211252-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse PPP2R2B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP2R2B antibody AAA211252 (lot AAA211252-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PPP2R2B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP2R2B antibody AAA211252 (lot AAA211252-2) used for IP at 6 ug per reaction. PPP2R2B was also immunoprecipitated by rabbit anti-PPP2R2B antibody BL4250. For blotting immunoprecipitated PPP2R2B, AAA211252 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PPP2R2B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP2R2B antibody AAA211252 (lot AAA211252-2) used for IP at 6 ug per reaction. PPP2R2B was also immunoprecipitated by rabbit anti-PPP2R2B antibody BL4250. For blotting immunoprecipitated PPP2R2B, AAA211252 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-PPP2R2B antibody
PP2A is a major serine/threonine phosphatase that is implicated in the negative control of cell cycle progression. It is a trimeric holoenzyme that is comprised of a catalytic, structural, and regulatory subunit. PPP2R2B is a regulatory subunit of PP2A. The regulatory subunits of PP2A are grouped into 3 families: B/PR55, B'/PR61, and B''/PR72. PPP2R2B encodes a beta isoform of the B/PR55 subunit family. PPP2R2B is brain specific. An expansion of a CAG repeat in the 5'region of PP2R2B is associated with spinocerebellar ataxia type 12 (SCA12), an autosomal dominant neurodegenerative disorder.
Product Categories/Family for anti-PPP2R2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,505 Da
NCBI Official Synonym Full Names
protein phosphatase 2, regulatory subunit B, beta
NCBI Official Symbol
PPP2R2B
NCBI Official Synonym Symbols
PR52B; SCA12; B55BETA; PR55BETA; PP2ABBETA; PP2APR55B; PR2ABBETA; PR55-BETA; PP2AB55BETA; PR2AB55BETA; PP2APR55BETA; PR2APR55BETA
NCBI Protein Information
serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
UniProt Gene Name
PPP2R2B
UniProt Entry Name
2ABB_HUMAN
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Product Notes
The PPP2R2B ppp2r2b (Catalog #AAA211252) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PPP2R2B Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPP2R2B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPP2R2B ppp2r2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP2R2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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