WB (Western Blot)
(Western blot analysis of lysate from SK-N-MC cell line, using PPP2R5B Antibody (C-term). AAA287758 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Rabbit anti-Human PPP2R5B Polyclonal Antibody | anti-PPP2R5B antibody
PPP2R5B Antibody (C-term)
Gene Names
PPP2R5B; B56B; PR61B
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PPP2R5B, Antibody; PPP2R5B Antibody (C-term); Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform; PP2A B subunit isoform B'-beta; PP2A B subunit isoform B56-beta; PP2A B subunit isoform PR61-beta; PP2A B subunit isoform R5-beta; PPP2R5B; anti-PPP2R5B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PPP2R5B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 466-500 amino acids from the C-terminal region of human PPP2R5B.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
494
Applicable Applications for anti-PPP2R5B antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysate from SK-N-MC cell line, using PPP2R5B Antibody (C-term). AAA287758 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from SK-N-MC cell line, using PPP2R5B Antibody (C-term). AAA287758 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
IF (Immunofluorescence)
(Fluorescent image of SH-SY5Y cells stained with PPP2R5B Antibody (C-term). AAA287758 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of SH-SY5Y cells stained with PPP2R5B Antibody (C-term). AAA287758 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-PPP2R5B antibody
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues.
Product Categories/Family for anti-PPP2R5B antibody
References
McCright B.,et al.J. Biol. Chem. 270:26123-26128(1995).
Zolnierowicz S.,et al.Biochem. J. 317:187-194(1996).
McCright B.,et al.J. Biol. Chem. 271:22081-22089(1996).
Kitajima T.S.,et al.Nature 441:46-52(2006).
Rodgers J.T.,et al.Mol. Cell 41:471-479(2011).
Zolnierowicz S.,et al.Biochem. J. 317:187-194(1996).
McCright B.,et al.J. Biol. Chem. 271:22081-22089(1996).
Kitajima T.S.,et al.Nature 441:46-52(2006).
Rodgers J.T.,et al.Mol. Cell 41:471-479(2011).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57393
NCBI Official Full Name
serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
NCBI Official Synonym Full Names
protein phosphatase 2, regulatory subunit B', beta
NCBI Official Symbol
PPP2R5B
NCBI Official Synonym Symbols
B56B; PR61B
NCBI Protein Information
serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
UniProt Gene Name
PPP2R5B
UniProt Entry Name
2A5B_HUMAN
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Product Notes
The PPP2R5B ppp2r5b (Catalog #AAA287758) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP2R5B Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP2R5B can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the PPP2R5B ppp2r5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP2R5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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