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WB (Western Blot)
(Detection of mouse PPP4C by western blot. Samples: Whole cell lysate (10 ug) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4C antibody (AAA211171 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse PPP4C Polyclonal Antibody | anti-PPP4C antibody
Rabbit anti-PPP4C Antibody, Affinity Purified
Gene Names
PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PPP4C, Antibody; Rabbit anti-PPP4C Antibody, Affinity Purified; PPP4; protein phosphatase X, catalytic subunit; Protein phosphatase X; protein phosphatase 4 (formerly X), catalytic subunit; PPX; serine/threonine-protein phosphatase 4 catalytic subunit; PPH3; PP4C; PP4; epididymis secretory sperm binding protein; PP-X; Serine/threonine-protein phosphatase 4 catalytic subunit; protein phosphatase 4 catalytic subunit; anti-PPP4C antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PPP4C antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Rabbit, Orangutan
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse PPP4C by western blot. Samples: Whole cell lysate (10 ug) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4C antibody (AAA211171 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse PPP4C by western blot. Samples: Whole cell lysate (10 ug) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4C antibody (AAA211171 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PPP4C by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4C antibody (AAA211171 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PPP4C by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP4C antibody (AAA211171 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PPP4C by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 15% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP4C antibody (AAA211171 lot 4) used for IP at 6 ug per reaction. PPP4C was also immunoprecipitated by a previous lot of this antibody (AAA211171 lot 3) and a second antibody against a different epitope of PPP4C (A304-923A). For blotting immunoprecipitated PPP4C, AAA211171 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PPP4C by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 15% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP4C antibody (AAA211171 lot 4) used for IP at 6 ug per reaction. PPP4C was also immunoprecipitated by a previous lot of this antibody (AAA211171 lot 3) and a second antibody against a different epitope of PPP4C (A304-923A). For blotting immunoprecipitated PPP4C, AAA211171 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohiostchemistry)
(Detection of mouse PPP4C by immunohistochemistry. Sample: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PPP4C (AAA211171 Lot 4) used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse PPP4C by immunohistochemistry. Sample: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PPP4C (AAA211171 Lot 4) used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PPP4C by immunohistochemistry. Sample: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PPP4C (AAA211171 Lot 4) used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PPP4C by immunohistochemistry. Sample: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PPP4C (AAA211171 Lot 4) used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB)
Related Product Information for anti-PPP4C antibody
PPP4C, also known as PPX, is the catalytic subunit of the PP4 serine/threonine protein phosphatase. PP4 is a holoenzyme that is comprised of the PPP4C catalytic subunit in association with the PP4-R1 or PP4-R2 regulatory subunits. PP4 has been demonstrated to directly interact with and activate NF-kappaB. PP4 also has been demonstrated to interact with histone deacetylase HDAC3 to influence transcription and to serve a role in microtubule organization during mitosis. Signaling through the MAPK and JNK-1 pathway has also been demonstrated to involve PP4.
Product Categories/Family for anti-PPP4C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,080 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 4 catalytic subunit isoform 1
NCBI Official Synonym Full Names
protein phosphatase 4, catalytic subunit
NCBI Official Symbol
PPP4C
NCBI Official Synonym Symbols
PP4; PPX; PP-X; PP4C; PPH3; PPP4
NCBI Protein Information
serine/threonine-protein phosphatase 4 catalytic subunit; protein phosphatase 4 (formerly X), catalytic subunit; protein phosphatase X, catalytic subunit
UniProt Protein Name
Serine/threonine-protein phosphatase 4 catalytic subunit
UniProt Gene Name
PPP4C
UniProt Synonym Gene Names
PPP4; PPX; PP4C; Pp4; PP-X
UniProt Entry Name
PP4C_HUMAN
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Product Notes
The PPP4C ppp4c (Catalog #AAA211171) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PPP4C Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPP4C can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPP4C ppp4c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP4C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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