IP (Immunoprecipitation)
(PPP4C was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-PPP4C rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PPP4C rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size :35 kDa)
Rabbit anti-Human PPP4C Polyclonal Antibody | anti-PPP4C antibody
Anti-PPP4C Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PPP4C, Antibody; Anti-PPP4C Antibody, Rabbit Polyclonal; PPP4C Antibody, Rabbit PAb, Antigen Affinity Purified; protein phosphatase 4, catalytic subunit; Anti-PP-X Antibody; Anti-PP4 Antibody; Anti-PP4C Antibody; Anti-PPH3 Antibody; Anti-PPP4 Antibody; Anti-PPX Antibody; anti-PPP4C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PPP4C
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PPP4C antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human PPP4C fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PPP4C fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PPP4C was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-PPP4C rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PPP4C rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size :35 kDa)
IP (Immunoprecipitation)
(PPP4C was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-PPP4C rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PPP4C rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size :35 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PPP4C in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PPP4C in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-PPP4C rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:35 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-PPP4C rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:35 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-PPP4C antibody
The mammalian orthologue of Pph3p is the protein serine/threonine phosphatase Ppp4c, which is found in high molecular mass complexes bound to a regulatory subunit R2. Protein phosphates 4 (PP4), encoded by the ppp4c gene, is a ubiquitously expressed phosphatase that has been implicated in the regulation of cytokine signaling and lymphocyte survival; recent reports suggest that PP4 may be involved in pre-TCR signaling and B cell development. Ppp4c is a critical positive regulator of BMP/Smad signaling during embryonic dorsoventral pattern formation in zebrafish. Ppp4c is required for the organization of the actin cytoskeleton at the leading edge of human cells during migration. The active forms of the RhoGTPases, Rac1, and Cdc42 are substantially decreased in the presence and absence of growth factor in Ppp4c depleted cells, implicating Ppp4c in the regulation of these GTPases.
Product Categories/Family for anti-PPP4C antibody
References
Martin-Granados C, et al. (2008) Depletion of protein phosphatase 4 in human cells reveals essential roles in centrosome maturation, cell migration and the regulation of rho gtpases. Int J Biochem Cell Biol 40 (10): 2315-2332.Liao FH, et al. (2014) Protein phosphatase 4 is an essential positive regulator for treg development, function, and protective gut immunity. Cell Biosci 4 25.Hastie CJ, et al. (2006) The saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit r2 confers resistance to the anticancer drug cisplatin. Febs j 273 (14): 3322-3334.
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Product Notes
The PPP4C (Catalog #AAA256036) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPP4C Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP4C can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PPP4C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP4C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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