WB (Western Blot)
(Detection of human PPP6C by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, Jurkat, U2OS, GaMG, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP6C antibody (AAA211176 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse PPP6C Polyclonal Antibody | anti-PPP6C antibody
Rabbit anti-PPP6C Antibody, Affinity Purified
Gene Names
PPP6C; PP6; PP6C
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PPP6C, Antibody; Rabbit anti-PPP6C Antibody, Affinity Purified; PP6; PP6C; serine/threonine protein phosphatase catalytic subunit; serine/threonine-protein phosphatase 6 catalytic subunit; Serine/threonine-protein phosphatase 6 catalytic subunit; protein phosphatase 6 catalytic subunit; anti-PPP6C antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PPP6C antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 255 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PPP6C by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, Jurkat, U2OS, GaMG, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP6C antibody (AAA211176 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PPP6C by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, Jurkat, U2OS, GaMG, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP6C antibody (AAA211176 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse PPP6C by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, CT26, TCMK-1, NIH 3T3, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP6C antibody (AAA211176 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse PPP6C by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, CT26, TCMK-1, NIH 3T3, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP6C antibody (AAA211176 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PPP6C by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP6C antibody (AAA211176 lot 2) used for IP at 6 ug per reaction. PPP6C was also immunoprecipitated by a previous lot of this antibody (AAA211176 lot 1) and a second antibody against a different epitope of PPP6C (BL3570). For blotting immunoprecipitated PPP6C, AAA211176 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PPP6C by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP6C antibody (AAA211176 lot 2) used for IP at 6 ug per reaction. PPP6C was also immunoprecipitated by a previous lot of this antibody (AAA211176 lot 1) and a second antibody against a different epitope of PPP6C (BL3570). For blotting immunoprecipitated PPP6C, AAA211176 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-PPP6C antibody
PPP6C is the protein phosphatase 6 catalytic subunit that is a member of the large PPP family of serine/threonine protein phosphatases. PPP6C is a type-2A phosphatase and is structurally related to PP2A and PP4. PP6 has been shown to play a role in the cell cycle through its regulation of cyclin D1. PPP6C has also been implicated in the negative control of NF-kappaB and TAK1 activation.
Product Categories/Family for anti-PPP6C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,947 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 6 catalytic subunit isoform b
NCBI Official Synonym Full Names
protein phosphatase 6, catalytic subunit
NCBI Official Symbol
PPP6C
NCBI Official Synonym Symbols
PP6; PP6C
NCBI Protein Information
serine/threonine-protein phosphatase 6 catalytic subunit; serine/threonine protein phosphatase catalytic subunit
UniProt Protein Name
Serine/threonine-protein phosphatase 6 catalytic subunit
UniProt Gene Name
PPP6C
UniProt Synonym Gene Names
PPP6; PP6C
UniProt Entry Name
PPP6_HUMAN
Similar Products
Product Notes
The PPP6C ppp6c (Catalog #AAA211176) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PPP6C Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPP6C can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPP6C ppp6c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP6C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.