WB (Western Blot)
(Western blot analysis of PPT1 (arrow) using rabbit polyclonal PPT1 Antibody (A284). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPT1 gene.)
Rabbit PPT1 Polyclonal Antibody | anti-PPT1 antibody
PPT1 Antibody (C-term)
Gene Names
PPT1; PPT; CLN1; INCL
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PPT1, Antibody; PPT1 Antibody (C-term); Palmitoyl-protein thioesterase 1; PPT-1; Palmitoyl-protein hydrolase 1; PPT1; PPT; anti-PPT1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-300 amino acids from the C-terminal region of human PPT1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
269-300
Sequence Length
306
Applicable Applications for anti-PPT1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of PPT1 (arrow) using rabbit polyclonal PPT1 Antibody (A284). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPT1 gene.)
WB (Western Blot)
(Western blot analysis of PPT1 (arrow) using rabbit polyclonal PPT1 Antibody (A284). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPT1 gene.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PPT1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PPT1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of PPT1 Antibody (C-term) in 293 cell line lysates (35ug/lane). PPT1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PPT1 Antibody (C-term) in 293 cell line lysates (35ug/lane). PPT1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-PPT1 antibody
Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.
Product Categories/Family for anti-PPT1 antibody
References
Calero, G., et al., J. Biol. Chem. 278(39):37957-37964 (2003).
Hofmann, S.L., et al., Curr. Mol. Med. 2(5):423-437 (2002).
Weimer, J.M., et al., Neuromolecular Med. 1(2):111-124 (2002).
Lu, J.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 93(19):10046-10050 (1996).
Crews, C.M., et al., Proc. Natl. Acad. Sci. U.S.A. 93(9):4316-4319 (1996).
Hofmann, S.L., et al., Curr. Mol. Med. 2(5):423-437 (2002).
Weimer, J.M., et al., Neuromolecular Med. 1(2):111-124 (2002).
Lu, J.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 93(19):10046-10050 (1996).
Crews, C.M., et al., Proc. Natl. Acad. Sci. U.S.A. 93(9):4316-4319 (1996).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34193
NCBI Official Full Name
palmitoyl-protein thioesterase 1 isoform 1
NCBI Official Synonym Full Names
palmitoyl-protein thioesterase 1
NCBI Official Symbol
PPT1
NCBI Official Synonym Symbols
PPT; CLN1; INCL
NCBI Protein Information
palmitoyl-protein thioesterase 1
UniProt Protein Name
Palmitoyl-protein thioesterase 1
UniProt Gene Name
PPT1
UniProt Synonym Gene Names
PPT; PPT-1
UniProt Entry Name
PPT1_HUMAN
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Product Notes
The PPT1 ppt1 (Catalog #AAA286463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 269-300. The PPT1 Antibody (C-term) reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's PPT1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PPT1 ppt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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