FCM/FACS (Flow Cytometry)
(PREX1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human PREX1 Polyclonal Antibody | anti-PREX1 antibody
PREX1 Antibody (C-term)
Gene Names
PREX1; P-REX1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PREX1, Antibody; PREX1 Antibody (C-term); Phosphatidylinositol 3,5-trisphosphate-dependent Rac exchanger 1 protein; P-Rex1; PtdIns(3,5)-dependent Rac exchanger 1; PREX1; KIAA1415; anti-PREX1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PREX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134-1163 amino acids from the C-terminal region of human PREX1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.42 (varies by lot)
Sequence Positions
1134-1163
Sequence Length
1659
Applicable Applications for anti-PREX1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PREX1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PREX1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(PREX1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PREX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PREX1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PREX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PREX1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PREX1 antibody detected the PREX1 protein (arrow).)
WB (Western Blot)
(PREX1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PREX1 antibody detected the PREX1 protein (arrow).)
Related Product Information for anti-PREX1 antibody
The protein encoded by this gene acts as a guanine
nucleotide exchange factor for the RHO family of small GTP-binding
proteins (RACs). It has been shown to bind to and activate RAC1 by
exchanging bound GDP for free GTP. The encoded protein, which is
found mainly in the cytoplasm, is activated by
phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma
subunits of heterotrimeric G proteins.
nucleotide exchange factor for the RHO family of small GTP-binding
proteins (RACs). It has been shown to bind to and activate RAC1 by
exchanging bound GDP for free GTP. The encoded protein, which is
found mainly in the cytoplasm, is activated by
phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma
subunits of heterotrimeric G proteins.
Product Categories/Family for anti-PREX1 antibody
References
Lewis, J.P., et al. Genomics 96(4):211-219(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Nie, B., et al. Cell. Signal. 22(5):770-782(2010)
Carretero-Ortega, J., et al. Mol. Pharmacol. 77(3):435-442(2010)
Ledezma-Sanchez, B.A., et al. Biochem. Biophys. Res. Commun. 391(4):1647-1652(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Nie, B., et al. Cell. Signal. 22(5):770-782(2010)
Carretero-Ortega, J., et al. Mol. Pharmacol. 77(3):435-442(2010)
Ledezma-Sanchez, B.A., et al. Biochem. Biophys. Res. Commun. 391(4):1647-1652(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
186203
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
NCBI Official Synonym Full Names
phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
NCBI Official Symbol
PREX1
NCBI Official Synonym Symbols
P-REX1
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
UniProt Gene Name
PREX1
UniProt Synonym Gene Names
KIAA1415; P-Rex1; PtdIns(3,4,5)-dependent Rac exchanger 1
UniProt Entry Name
PREX1_HUMAN
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Product Notes
The PREX1 prex1 (Catalog #AAA287459) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1134-1163. The PREX1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PREX1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PREX1 prex1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PREX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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