IP (Immunoprecipitation)
(ZFP42 was immunoprecipitated using:Lane A:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-ZFP42 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ZFP42 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 42 kDa)
Rabbit anti-Human PREX1 Polyclonal Antibody | anti-PREX1 antibody
Anti-PREX1 Antibody, Rabbit Polyclonal
Gene Names
PREX1; P-REX1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PREX1, Antibody; Anti-PREX1 Antibody, Rabbit Polyclonal; PREX1 Antibody, Rabbit PAb, Antigen Affinity Purified; phosphatidylinositol-3, 4, 5-trisphosphate-dependent Rac exchange factor 1; Anti-P-REX1 Antibody; anti-PREX1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PREX1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PREX1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the human PREX1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the human PREX1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(ZFP42 was immunoprecipitated using:Lane A:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-ZFP42 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ZFP42 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 42 kDa)
IP (Immunoprecipitation)
(ZFP42 was immunoprecipitated using:Lane A:0.5 mg Raw264.7 Whole Cell Lysate2 uL anti-ZFP42 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ZFP42 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 42 kDa)
WB (Western Blot)
(Anti-PREX1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:42 kDa)
WB (Western Blot)
(Anti-PREX1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:42 kDa)
Related Product Information for anti-PREX1 antibody
PREX1 is a Rac guanine exchange factor that coordinates signaling inputs from G protein-coupled receptors and receptor tyrosine kinases (RTKs). PREX1 creates a positive feedback loop to drive RTK, phosphatidylinositol 3-kinase (PI3K)/AKT, and MEK/ERK signaling. High PREX1 levels predict sensitivity to PI3K inhibitors in breast cancer cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
175,847 Da
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
NCBI Official Synonym Full Names
phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
NCBI Official Symbol
PREX1
NCBI Official Synonym Symbols
P-REX1
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein; ptdIns(3,4,5)-dependent Rac exchanger 1
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
UniProt Gene Name
PREX1
UniProt Synonym Gene Names
KIAA1415; P-Rex1; PtdIns(3,4,5)-dependent Rac exchanger 1
UniProt Entry Name
PREX1_HUMAN
Similar Products
Product Notes
The PREX1 prex1 (Catalog #AAA254491) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PREX1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PREX1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PREX1 prex1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PREX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.