PRICKLE2 Polyclonal Antibody | anti-PRICKLE2 antibody

Anti-PRICKLE2 Antibody (Internal) IHC-plus

Cat. Num. AAA162151

• Gene Names: PRICKLE2; EPM5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Peptide column affinity chromatography
• Synonyms: PRICKLE2, Antibody; Anti-PRICKLE2 Antibody (Internal) IHC-plus; Rabbit Polyclonal to Human PRICKLE2; Human PRICKLE2; EPM5; Prickle homolog 2 (Drosophila); Prickle-like protein 2; Prickle-like 2 (Drosophila); anti-PRICKLE2 antibody

• Host: PRICKEL2 antibody was produced in rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Two isoforms of PRIKCLE2 are known to exist. PRICKLE2 antibody is predicted to no cross-react with other PRICKLE protien family members.
• Purity/Purification: Peptide column affinity chromatography
• Form/Format: PBS containing 0.02% sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PRICKLE2 antibody: ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
• Target: PRICKLE2
• Immunogen Species: PRICKLE2 antibody was raised against Human
• Antigen Type: Synthetic peptide
• Immunogen: PRICKLE2 antibody was raised against a 15 AA synthetic peptide near the center of human PRICKLE2. The immunogen is located within aa 420-470 of PRICKLE2.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for up to 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

WB (Western Blot)

(Western blot analysis of PRICKLE2 in A-20 lysate with PRICKLE2 antibody at 1 ug/ml.)

IF (Immunofluorescence)

(Immunofluorescence of PRICKLE2 in rat brain cells with PRICKLE2 antibody at 20 µg/mL.)

IHC (Immunohistochemistry)

(Anti-PRICKLE2 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-PRICKLE2 antibody

PRICKLE2 Antibody, EPM5 Antibody, Prickle homolog 2 (Drosophila) Antibody, Prickle-like protein 2 Antibody, Prickle-like 2 (Drosophila) Antibody Description: PRICKLE2 encodes a homolog of Drosophila prickle. The exact function of this gene is not known, however, studies in mice suggest that it may be involved in seizure prevention. Mutations in this gene are associated with progressive myoclonic epilepsy type 5.

NCBI and Uniprot Product Information

• NCBI GI #: 38524620
• NCBI GeneID: 166336
• NCBI Accession #: NP_942559.1
• NCBI GenBank Nucleotide #: NM_198859.3
• UniProt Accession #: Q7Z3G6; Q0VF44
• NCBI Official Full Name: prickle-like protein 2
• NCBI Official Synonym Full Names: prickle planar cell polarity protein 2
• NCBI Official Symbol: PRICKLE2
• NCBI Official Synonym Symbols: EPM5
• NCBI Protein Information: prickle-like protein 2
• UniProt Protein Name: Prickle-like protein 2
• UniProt Gene Name: PRICKLE2
• UniProt Entry Name: PRIC2_HUMAN