Rabbit PRK1/2 Polyclonal Antibody | anti-PRK1/2 antibody

Phospho-PRK1/2 (Thr774/Thr816) Antibody

Cat. Num. AAA329855

• Gene Names: PKN1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
• Reactivity: Human, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (88%), Xenopus (100%)
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: PRK1/2, Antibody; Phospho-PRK1/2 (Thr774/Thr816) Antibody; DBK; PAK 1; PAK-1; PAK1; PKC1; PKN ALPHA; PKN; Pkn1; PKN1_HUMAN; PRK1; PRKCL1; Protease activated kinase 1; Protease-activated kinase 1; Protein kinase C like 1; Protein kinase C like PKN; Protein kinase C related kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase N1; Protein kinase PKN alpha; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine threonine kinase N; Serine threonine protein kinase N; Serine-threonine protein kinase N; Serine/threonine protein kinase N1; Serine/threonine-protein kinase N1; Cardiolipin activated protein kinase Pak2; MGC150606; MGC71074; PAK 2; PAK2; PKN gamma; PKN2; PKN2_HUMAN; PRK2; PRKCL2; PRO2042; Protein kinase C like 2; Protein kinase C related kinase 2; Protein kinase C-like 2; Protein kinase N2; Protein-kinase C-related kinase 2; Serine/threonine-protein kinase N2; anti-PRK1/2 antibody

• Host: Rabbit
• Reactivity: Human, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (88%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-PRK1/2 (Thr774/Thr816) Antibody detects endogenous levels of PRK1/2 only when phosphorylated at Thr774/816.; Tissue Specificity: Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PRK1/2 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human PRK1/2 around the phosphorylation site of Thr774/816.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Autophosphorylated. Phosphorylated during mitosis. Phosphorylated by CDK10. Activated by limited proteolysis with trypsin (By similarity). Proteolytically cleaved by caspase-3 during the induction of apoptotic cell death.
• Subunit Structure: Interacts (via the REM repeats) with RHOA (GTP-bound form preferentially) and interacts (via the REM repeats) with RAC1 (GTP-bound form preferentially); the interactions induce its autophosphorylation. Interacts with RHOC. Interacts with NCK1 and NCK2. Interacts with NCK1 (via SH3 domains) (By similarity). Interacts with CD44. Interacts (via C-terminal kinase domain) with PDPK1; the interaction stimulates PDPK1 kinase activity. Interacts with MAP3K2; the interaction activates PRK2 kinase activity in a MAP3K2-independent kinase activity. Interacts (via C-terminal domain) with AKT1; the interaction occurs with the C-terminal cleavage product of PRK2 in apoptotic cells. Interacts (via C-terminus) with PTPN13 (via PDZ 3 domain). Interacts with CDK10. (Microbial infection) Interacts with HCV NS5B (via N-terminal finger domain).
• Similarity: The N-terminal regioninterferes with the interaction between AKT1 and the C-terminal regionof PKN2.The C1 domain does not bind the diacylglycerol (DAG).The apoptotic C-terminal cleavage product inhibits EGF-induced kinase activity of AKT1 phosphorylation at 'Thr-308' and 'Ser-473' sites, PDPK1 autophosphorylation and kinases PRKCD and PRKCZ phosphorylations.Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
• Subcellular Location: Cytoplasm. Nucleus. Membrane. Cell projection>Lamellipodium. Cytoplasm>Cytoskeleton. Cleavage furrow. Midbody. Cell junction.; Note: Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohiostchemistry)

(Staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of PRK1/2 (Phospho-Thr774/816) using Na2VO3 treated HepG2 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-PRK1/2 antibody

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis. (Microbial infection) Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.

NCBI and Uniprot Product Information

• NCBI GI #: 47132589
• NCBI GeneID: 5585
• NCBI Accession #: NP_002732.3
• NCBI GenBank Nucleotide #: NM_002741.3
• UniProt Accession #: Q16512; Q16513
• Molecular Weight: 103,932 Da
• NCBI Official Full Name: serine/threonine-protein kinase N1 isoform 2
• NCBI Official Synonym Full Names: protein kinase N1
• NCBI Official Symbol: PKN1
• NCBI Official Synonym Symbols: DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
• NCBI Protein Information: serine/threonine-protein kinase N1; protein kinase C-like 1; protein kinase PKN-alpha; protein kinase C-like PKN; serine-threonine kinase N; protease-activated kinase 1; protein kinase C-related kinase 1; protein-kinase C-related kinase 1; serine-threonin
• UniProt Protein Name: Serine/threonine-protein kinase N1
• UniProt Gene Name: PKN1
• UniProt Synonym Gene Names: PAK1; PKN; PRK1; PRKCL1; PAK-1
• UniProt Entry Name: PKN1_HUMAN