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IHC (Immunohistochemistry)
(PRKAR1A Antibody-Immunohistochemistry of paraffin-embedded human esophageal cancer using PRKAR1A antibody at dilution of 1:100 (200x lens).)
Rabbit anti-Human PRKAR1A Polyclonal Antibody | anti-PRKAR1A antibody
PRKAR1A Rabbit anti-Human Polyclonal Antibody
Gene Names
PRKAR1A; CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Affinity purified
Synonyms
PRKAR1A; Polyclonal Antibody; PRKAR1A Rabbit anti-Human Polyclonal Antibody; IHC-plus PRKAR1A Antibody; ADOHR; CNC; ACRDYS1; TSE1; PRKAR1; Tissue specific extinguisher 1; Tissue-specific extinguisher 1; CAR; CNC1; PKR1; PPNAD1; anti-PRKAR1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Antibody has not been tested for cross reactivity with PRKAR1B.
Purity/Purification
Affinity purified
Form/Format
PBS, pH7.3, 0.02% Sodium Azide, 50% Glycerol
Applicable Applications for anti-PRKAR1A antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Application Notes
IHC: 1:50-1:200
IHC-P: 1:200
WB: 1:500-1:2000
The predicted MW is 38kDa/42kDa, while the observed MW by Western blot was 45kDa.
IHC-P: 1:200
WB: 1:500-1:2000
The predicted MW is 38kDa/42kDa, while the observed MW by Western blot was 45kDa.
Target
Human PRKAR1A
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PRKAR1A (NP_002725.1). MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFL
Conjugation
Unconjugated
Family
Protein Kinase Non catalytic
Subfamily
CAMP
Preparation and Storage
Store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistochemistry)
(PRKAR1A Antibody-Immunohistochemistry of paraffin-embedded human esophageal cancer using PRKAR1A antibody at dilution of 1:100 (200x lens).)
IHC (Immunohistochemistry)
(PRKAR1A Antibody-Immunohistochemistry of paraffin-embedded human esophageal cancer using PRKAR1A antibody at dilution of 1:100 (200x lens).)
IHC (Immunohistochemistry)
(PRKAR1A Antibody-Immunohistochemistry of paraffin-embedded human esophagus using PRKAR1A antibody at dilution of 1:100 (200x lens).)
IHC (Immunohistochemistry)
(PRKAR1A Antibody-Immunohistochemistry of paraffin-embedded human esophagus using PRKAR1A antibody at dilution of 1:100 (200x lens).)
IHC (Immunohistchemistry)
(PRKAR1A Antibody-Immunohistochemistry of paraffin-embedded human oophoroma tissue.)
IHC (Immunohistchemistry)
(PRKAR1A Antibody-Immunohistochemistry of paraffin-embedded human oophoroma tissue.)
IHC (Immunohistochemistry)
(PRKAR1A Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(PRKAR1A Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(PRKAR1A Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(PRKAR1A Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(PRKAR1A Antibody-Western blot analysis of extracts of various cell lines using PRKAR1A antibody.)
WB (Western Blot)
(PRKAR1A Antibody-Western blot analysis of extracts of various cell lines using PRKAR1A antibody.)
WB (Western Blot)
(PRKAR1A Antibody-Western blot analysis of extracts of various cell lines, using PRKAR1A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)
WB (Western Blot)
(PRKAR1A Antibody-Western blot analysis of extracts of various cell lines, using PRKAR1A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)
ICC (Immunocytochemistry)
(PRKAR1A Antibody-Immunofluorescence analysis of HeLa cell using PRKAR1A antibody. Blue: DAPI for nuclear staining.)
ICC (Immunocytochemistry)
(PRKAR1A Antibody-Immunofluorescence analysis of HeLa cell using PRKAR1A antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-PRKAR1A antibody
PRKAR1A antibody is an unconjugated rabbit polyclonal antibody to human PRKAR1A. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,003 Da
NCBI Official Full Name
Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, alpha (PRKAR1A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
protein kinase, cAMP-dependent, regulatory, type I, alpha
NCBI Official Symbol
PRKAR1A
NCBI Official Synonym Symbols
CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
NCBI Protein Information
cAMP-dependent protein kinase type I-alpha regulatory subunit; Carney complex type 1; cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; protein kinase A type 1a regulatory subunit; tissu
UniProt Protein Name
cAMP-dependent protein kinase type I-alpha regulatory subunit
UniProt Gene Name
PRKAR1A
UniProt Synonym Gene Names
PKR1; PRKAR1; TSE1; TSE1
UniProt Entry Name
KAP0_HUMAN
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Product Notes
The PRKAR1A prkar1a (Catalog #AAA21298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRKAR1A Rabbit anti-Human Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRKAR1A can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). IHC: 1:50-1:200 IHC-P: 1:200 WB: 1:500-1:2000 The predicted MW is 38kDa/42kDa, while the observed MW by Western blot was 45kDa. Researchers should empirically determine the suitability of the PRKAR1A prkar1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKAR1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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