FCM/FACS (Flow Cytometry)
(PRM3 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, Mouse PRM3 Polyclonal Antibody | anti-PRM3 antibody
PRM3 Antibody (C-term)
Reactivity
Human, Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PRM3, Antibody; PRM3 Antibody (C-term); Protamine-3; Sperm protamine P3; PRM3; anti-PRM3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This PRM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the C-terminal region of human PRM3.
Purity/Purification
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-PRM3 antibody
ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Function
Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex (By similarity).
Cellular Location
Nucleus. Chromosome.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PRM3 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PRM3 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(PRM3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRM3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PRM3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRM3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of PRM3 Antibody (C-term) in Jurkat cell line lysates (35ug/lane).PRM3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PRM3 Antibody (C-term) in Jurkat cell line lysates (35ug/lane).PRM3 (arrow) was detected using the purified Pab.)
Related Product Information for anti-PRM3 antibody
PRM3 Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex
Product Categories/Family for anti-PRM3 antibody
References
Imken, L., et al. Mol. Hum. Reprod. 15(11):733-738(2009) Oliva, R. Hum. Reprod. Update 12(4):417-435(2006) Kramer, J.A., et al. Comp. Biochem. Physiol. C, Pharmacol. Toxicol. Endocrinol. 120(3):467-473(1998)
NCBI and Uniprot Product Information
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Product Notes
The PRM3 prm3 (Catalog #AAA296708) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRM3 Antibody (C-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRM3 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PRM3 prm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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