IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit PRMT1 Polyclonal Antibody | anti-PRMT1 antibody
PRMT1 Antibody (C-term)
Gene Names
PRMT1; ANM1; HCP1; IR1B4; HRMT1L2
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PRMT1, Antibody; PRMT1 Antibody (C-term); Protein arginine N-methyltransferase 1; 211-; Histone-arginine N-methyltransferase PRMT1; Interferon receptor 1-bound protein 4; PRMT1; HMT2; HRMT1L2; IR1B4; anti-PRMT1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PRMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-347 amino acids from the C-terminal region of human PRMT1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
316-347
Sequence Length
353
Applicable Applications for anti-PRMT1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of PRMT1 (arrow) using rabbit polyclonal PRMT1 Antibody (C-term) (RB00005). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of PRMT1 (arrow) using rabbit polyclonal PRMT1 Antibody (C-term) (RB00005). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT1 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-PRMT1 antibody
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
Product Categories/Family for anti-PRMT1 antibody
References
Zhang, X., et al., EMBO J. 19(14):3509-3519 (2000).
Scorilas, A., et al., Biochem. Biophys. Res. Commun. 278(2):349-359 (2000).
Scott, H.S., et al., Genomics 48(3):330-340 (1998).
Abramovich, C., et al., EMBO J. 16(2):260-266 (1997).
Nikawa, J., et al., Gene 171(1):107-111 (1996).
Scorilas, A., et al., Biochem. Biophys. Res. Commun. 278(2):349-359 (2000).
Scott, H.S., et al., Genomics 48(3):330-340 (1998).
Abramovich, C., et al., EMBO J. 16(2):260-266 (1997).
Nikawa, J., et al., Gene 171(1):107-111 (1996).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41516
NCBI Official Full Name
protein arginine N-methyltransferase 1 isoform 1
NCBI Official Synonym Full Names
protein arginine methyltransferase 1
NCBI Official Symbol
PRMT1
NCBI Official Synonym Symbols
ANM1; HCP1; IR1B4; HRMT1L2
NCBI Protein Information
protein arginine N-methyltransferase 1
UniProt Protein Name
Protein arginine N-methyltransferase 1
UniProt Gene Name
PRMT1
UniProt Synonym Gene Names
HMT2; HRMT1L2; IR1B4
UniProt Entry Name
ANM1_HUMAN
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Product Notes
The PRMT1 prmt1 (Catalog #AAA289811) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 316-347. The PRMT1 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PRMT1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PRMT1 prmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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