IP (Immunoprecipitation)
(Immunoprecipitating PDCD4 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA119711 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA119711(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
Rabbit anti-Human Programmed cell death protein 4 Polyclonal Antibody | anti-PDCD4 antibody
Rabbit anti-human Programmed cell death protein 4 polyclonal Antibody(PDCD4)
Gene Names
PDCD4; H731
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
Programmed cell death protein 4, Antibody; Rabbit anti-human Programmed cell death protein 4 polyclonal Antibody(PDCD4); Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a; PDCD4; H731; anti-PDCD4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
469
Applicable Applications for anti-PDCD4 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Programmed cell death protein 4 protein (1-260aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-27122 / sc-27123 / sc-292504
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating PDCD4 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA119711 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA119711(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating PDCD4 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA119711 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA119711(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA119711 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA119711 at dilution 1:100)
WB (Western Blot)
(All lanes: Programmed cell death protein 4 antibody at 4ug/mlLane 1:Hela whole cell lysateLane 2:MCF-7 whole cell lysateLane 3:jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 52,51 kDaObserved band size: 52 kDaImmunohisochemistry)
WB (Western Blot)
(All lanes: Programmed cell death protein 4 antibody at 4ug/mlLane 1:Hela whole cell lysateLane 2:MCF-7 whole cell lysateLane 3:jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 52,51 kDaObserved band size: 52 kDaImmunohisochemistry)
Related Product Information for anti-PDCD4 antibody
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,576 Da
NCBI Official Full Name
programmed cell death protein 4 isoform 1
NCBI Official Synonym Full Names
programmed cell death 4 (neoplastic transformation inhibitor)
NCBI Official Symbol
PDCD4
NCBI Official Synonym Symbols
H731
NCBI Protein Information
programmed cell death protein 4
UniProt Protein Name
Programmed cell death protein 4
UniProt Gene Name
PDCD4
UniProt Synonym Gene Names
H731
UniProt Entry Name
PDCD4_HUMAN
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Product Notes
The PDCD4 pdcd4 (Catalog #AAA119711) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Programmed cell death protein 4 polyclonal Antibody(PDCD4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Programmed cell death protein 4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PDCD4 pdcd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Programmed cell death protein 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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