IP (Immunoprecipitation)
(PRKD2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate4 uL anti-PRKD2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRKD2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size: 100 kDa)
Rabbit anti-Human Protein Kinase D2/PRKD2 Polyclonal Antibody | anti-PRKD2 antibody
Anti-Protein Kinase D2/PRKD2 Antibody, Rabbit Polyclonal
Gene Names
PRKD2; PKD2; HSPC187; nPKC-D2
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Protein Kinase D2/PRKD2, Antibody; Anti-Protein Kinase D2/PRKD2 Antibody, Rabbit Polyclonal; PRKD2 Antibody, Rabbit PAb, Antigen Affinity Purified; protein kinase D2; Anti-HSPC187 Antibody; Anti-nPKC-D2 Antibody; Anti-PKD2 Antibody; anti-PRKD2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PRKD2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PRKD2 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human PRKD2 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human PRKD2 (rh PRKD2). PRKD2 specific IgG was purified by Human PRKD2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PRKD2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate4 uL anti-PRKD2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRKD2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size: 100 kDa)
IP (Immunoprecipitation)
(PRKD2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate4 uL anti-PRKD2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRKD2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size: 100 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of PRKD2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PRKD2 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of PRKD2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PRKD2 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)
WB (Western Blot)
(Anti-PRKD2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:97 kDaObserved band size:113 kDa)
WB (Western Blot)
(Anti-PRKD2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:97 kDaObserved band size:113 kDa)
Related Product Information for anti-PRKD2 antibody
Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein that belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2/PKD2 is widely expressed. It contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. PRKD2/PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-71 and phosphorylation of Ser-76 by PKC relieves auto-inhibition by the PH domain. PRKD2/PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
References
Zhang QH, et al., 2000, Genome Res 10 (10): 1546-60. Di Bernardo MC, et al., 2008, Nature genetics. 40 (10) :1204-10. Zhang L, et al., 2009, American journal of nephrology. 30 (6) :505-13.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,143 Da
NCBI Official Full Name
serine/threonine-protein kinase D2 isoform A
NCBI Official Synonym Full Names
protein kinase D2
NCBI Official Symbol
PRKD2
NCBI Official Synonym Symbols
PKD2; HSPC187; nPKC-D2
NCBI Protein Information
serine/threonine-protein kinase D2
UniProt Protein Name
Serine/threonine-protein kinase D2
UniProt Gene Name
PRKD2
UniProt Synonym Gene Names
PKD2
UniProt Entry Name
KPCD2_HUMAN
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Product Notes
The PRKD2 prkd2 (Catalog #AAA255458) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Protein Kinase D2/PRKD2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase D2/PRKD2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PRKD2 prkd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Kinase D2/PRKD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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