WB (Western Blot)
(Western blot analysis of PPP1CB (arrow) using rabbit polyclonal PPP1CB Antibody (K301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene.)
Rabbit Protein Phosphatase 1 beta (PPP1CB) Polyclonal Antibody | anti-PPP1CB antibody
Protein Phosphatase 1 beta (PPP1CB) Antibody (C-term)
Gene Names
PPP1CB; PP1B; PP-1B; PPP1CD; PP1beta; HEL-S-80p
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus)
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Protein Phosphatase 1 beta (PPP1CB), Antibody; Protein Phosphatase 1 beta (PPP1CB) Antibody (C-term); Serine/threonine-protein phosphatase PP1-beta catalytic subunit; PP-1B; PPP1CD; PPP1CB; anti-PPP1CB antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Protein Phosphatase 1 beta (PPP1CB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human Protein Phosphatase 1 beta (PPP1CB).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
286-315
Sequence Length
327
Applicable Applications for anti-PPP1CB antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of PPP1CB (arrow) using rabbit polyclonal PPP1CB Antibody (K301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene.)
WB (Western Blot)
(Western blot analysis of PPP1CB (arrow) using rabbit polyclonal PPP1CB Antibody (K301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene.)
IF (Immunofluorescence)
(Fluorescent image of U251 cell stained with PPP1CB Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PPP1CB primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent image of U251 cell stained with PPP1CB Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PPP1CB primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Related Product Information for anti-PPP1CB antibody
Protein phosphatase-1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases involved in the dephosphorylation of a variety of proteins. These enzymes work in opposition to the protein kinases to control the level of phosphorylation. Protein phosphatase (PP1) is essential for cell division, and it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis, as well as in regulation of ionic conductances and long-term synaptic plasticity. PP1 has 3 catalytic subunits, designated alpha (PPP1CA), beta (PPP1CB), and gamma (PPP1CC).
Product Categories/Family for anti-PPP1CB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37187
NCBI Official Full Name
serine/threonine-protein phosphatase PP1-beta catalytic subunit isoform 1
NCBI Official Synonym Full Names
protein phosphatase 1, catalytic subunit, beta isozyme
NCBI Official Symbol
PPP1CB
NCBI Official Synonym Symbols
PP1B; PP-1B; PPP1CD; PP1beta; HEL-S-80p
NCBI Protein Information
serine/threonine-protein phosphatase PP1-beta catalytic subunit
UniProt Protein Name
Serine/threonine-protein phosphatase PP1-beta catalytic subunit
UniProt Gene Name
PPP1CB
UniProt Synonym Gene Names
PP-1B; PPP1CD
UniProt Entry Name
PP1B_HUMAN
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Product Notes
The PPP1CB ppp1cb (Catalog #AAA285716) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 286-315. The Protein Phosphatase 1 beta (PPP1CB) Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Phosphatase 1 beta (PPP1CB) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the PPP1CB ppp1cb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Phosphatase 1 beta (PPP1CB), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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