WB (Western Blot)
(Detection of human and mouse PRP6 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PRP6 antibody AAA212011 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
Rabbit anti-Human, Mouse PRP6 Polyclonal Antibody | anti-PRP6 antibody
Rabbit anti-PRP6 Antibody, Affinity Purified
Gene Names
PRPF6; TOM; ANT1; Prp6; RP60; ANT-1; hPrp6; U5-102K; C20orf14; SNRNP102
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PRP6, Antibody; Rabbit anti-PRP6 Antibody, Affinity Purified; PRP6 pre-mRNA processing factor 6 homolog; U5-102K; U5-102 kDa protein; U5 snRNP-associated 102 kDa protein; TOM; ANT1; SNRNP102; RP60; putative mitochondrial outer membrane protein import receptor; Prp6; pre-mRNA-processing factor 6; p102 U5 small nuclear ribonucleoprotein particle-binding protein; hPrp6; ANT-1; C20orf14; Androgen receptor N-terminal domain-transactivating protein 1; androgen receptor N-terminal domain transactivating protein-1; PRP6 homolog; Pre-mRNA-processing factor 6; pre-mRNA processing factor 6; anti-PRP6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PRP6 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 150 and 200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse PRP6 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PRP6 antibody AAA212011 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
WB (Western Blot)
(Detection of human and mouse PRP6 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PRP6 antibody AAA212011 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
IHC (Immunohistochemistry)
(Detection of human PRP6 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 (Cat. No. AAA212011 Lot1) used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PRP6 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 (Cat. No. AAA212011 Lot1) used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB)
Related Product Information for anti-PRP6 antibody
Pre-mRNA-processing factor 6 (PRP6) is involved in pre-mRNA splicing. PRP6 may act in the tri-snRNP complex as a bridging factor between U5 and U4/U6 snRNPs in the late step of spliceosome assembly. It may be necessary for tri-snRNP formation [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/O94906].
Product Categories/Family for anti-PRP6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
102,429 Da
NCBI Official Full Name
pre-mRNA-processing factor 6
NCBI Official Synonym Full Names
pre-mRNA processing factor 6
NCBI Official Symbol
PRPF6
NCBI Official Synonym Symbols
TOM; ANT1; Prp6; RP60; ANT-1; hPrp6; U5-102K; C20orf14; SNRNP102
NCBI Protein Information
pre-mRNA-processing factor 6
UniProt Protein Name
Pre-mRNA-processing factor 6
UniProt Gene Name
PRPF6
UniProt Synonym Gene Names
C20orf14; ANT-1; U5-102 kDa protein
UniProt Entry Name
PRP6_HUMAN
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Product Notes
The PRP6 prpf6 (Catalog #AAA212011) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PRP6 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRP6 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PRP6 prpf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRP6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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