WB (Western Blot)
(Detection of human PSMA1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMA1 antibody AAA212313 (lot AAA212313-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human PSMA1 Polyclonal Antibody | anti-PSMA1 antibody
Rabbit anti-PSMA1 Antibody, Affinity Purified
Gene Names
PSMA1; NU; HC2; PROS30; HEL-S-275
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PSMA1, Antibody; Rabbit anti-PSMA1 Antibody, Affinity Purified; PROS-30; proteasome subunit, alpha-type, 1; proteasome subunit nu; proteasome subunit alpha type-1; proteasome nu chain; testicular tissue protein Li 150; proteasome (prosome, macropain) subunit, alpha type, 1; protein P30-33K; NU; multicatalytic endopeptidase complex subunit C2; macropain subunit nu; macropain subunit C2; HEL-S-275; HC2; epididymis secretory protein Li 275; 30 kDa prosomal protein; proteasome component C2; PROS30; Proteasome subunit alpha type-1; proteasome subunit alpha 1; anti-PSMA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PSMA1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine, Orangutan
Immunogen
Between 213 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PSMA1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMA1 antibody AAA212313 (lot AAA212313-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PSMA1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMA1 antibody AAA212313 (lot AAA212313-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PSMA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSMA1 antibody AAA212313 (lot AAA212313-1) used for IP at 6 ug/mg lysate. PSMA1 was also immunoprecipitated, albeit at low efficiency, by rabbit anti-PSMA1 antibody For blotting immunoprecipitated PSMA1, AAA212313 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PSMA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSMA1 antibody AAA212313 (lot AAA212313-1) used for IP at 6 ug/mg lysate. PSMA1 was also immunoprecipitated, albeit at low efficiency, by rabbit anti-PSMA1 antibody For blotting immunoprecipitated PSMA1, AAA212313 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human PSMA1 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-PSMA1 (Cat. No. Lot1, left image and Cat. No. AAA212313 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PSMA1 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-PSMA1 (Cat. No. Lot1, left image and Cat. No. AAA212313 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-PSMA1 antibody
PSMA1 [proteasome (prosome, macropain) subunit, alpha type, 1] is a subunit of the proteasome, a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA1 is a member of the peptidase T1A family that is a 20S core alpha subunit [taken from NCBI Entrez Gene (Gene ID: 5682)].
Product Categories/Family for anti-PSMA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,239 Da
NCBI Official Full Name
proteasome subunit alpha type-1 isoform 2
NCBI Official Synonym Full Names
proteasome (prosome, macropain) subunit, alpha type, 1
NCBI Official Symbol
PSMA1
NCBI Official Synonym Symbols
NU; HC2; PROS30; HEL-S-275
NCBI Protein Information
proteasome subunit alpha type-1; 30 kDa prosomal protein; PROS-30; epididymis secretory protein Li 275; macropain subunit C2; macropain subunit nu; multicatalytic endopeptidase complex subunit C2; proteasome component C2; proteasome nu chain; proteasome s
UniProt Protein Name
Proteasome subunit alpha type-1
UniProt Gene Name
PSMA1
UniProt Synonym Gene Names
HC2; NU; PROS30; PSC2; PROS-30
UniProt Entry Name
PSA1_HUMAN
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Product Notes
The PSMA1 psma1 (Catalog #AAA212313) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PSMA1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMA1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PSMA1 psma1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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