WB (Western Blot)
(Detection of human PSMB2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMB2 antibody AAA212295 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
Rabbit anti-Human PSMB2 Polyclonal Antibody | anti-PSMB2 antibody
Rabbit anti-PSMB2 Antibody, Affinity Purified
Gene Names
PSMB2; HC7-I
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PSMB2, Antibody; Rabbit anti-PSMB2 Antibody, Affinity Purified; multicatalytic endopeptidase complex subunit C7-1; testicular tissue protein Li 152; proteasome subunit, beta type, 2; proteasome subunit beta type-2; proteasome component C7-I; proteasome beta 2 subunit; multicatalytic endopeptidase complex subunit C7-I; macropain subunit C7-I; HC7-I; proteasome (prosome, macropain) subunit, beta type, 2; Proteasome subunit beta type-2; proteasome subunit beta 2; anti-PSMB2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PSMB2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 151 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PSMB2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMB2 antibody AAA212295 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
WB (Western Blot)
(Detection of human PSMB2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMB2 antibody AAA212295 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).)
IHC (Immunohistochemistry)
(Detection of human PSMB2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PSMB2 (Cat. No. AAA212295 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human PSMB2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PSMB2 (Cat. No. AAA212295 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
Related Product Information for anti-PSMB2 antibody
Proteasome (prosome, macropain) subunit, alpha type, 2 (PSMA2) is a component of the proteasome, a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA2 is a member of the peptidase T1A family, that is a 20S core alpha subunit [taken from NCBI Entrez Gene (Gene ID: 5683)].
Product Categories/Family for anti-PSMB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,836 Da
NCBI Official Full Name
proteasome subunit beta type-2 isoform 1
NCBI Official Synonym Full Names
proteasome (prosome, macropain) subunit, beta type, 2
NCBI Official Symbol
PSMB2
NCBI Official Synonym Symbols
HC7-I
NCBI Protein Information
proteasome subunit beta type-2; macropain subunit C7-I; multicatalytic endopeptidase complex subunit C7-1; multicatalytic endopeptidase complex subunit C7-I; proteasome beta 2 subunit; proteasome component C7-I; proteasome subunit, beta type, 2
UniProt Protein Name
Proteasome subunit beta type-2
UniProt Gene Name
PSMB2
UniProt Entry Name
PSB2_HUMAN
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Product Notes
The PSMB2 psmb2 (Catalog #AAA212295) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PSMB2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMB2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PSMB2 psmb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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