FCM/FACS (Flow Cytometry)
(PSMB8 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human PSMB8 Polyclonal Antibody | anti-PSMB8 antibody
PSMB8 Antibody (N-term)
Gene Names
PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PSMB8, Antibody; PSMB8 Antibody (N-term); Proteasome subunit beta type-8; Low molecular mass protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; Proteasome component C13; Proteasome subunit beta-5i; Really interesting new gene 10 protein; PSMB8; LMP7; PSMB5i; RING10; Y2; anti-PSMB8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PSMB8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-36 amino acids from the N-terminal region of human PSMB8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.41 (varies by lot)
Sequence Positions
8-36
Sequence Length
272
Applicable Applications for anti-PSMB8 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PSMB8 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PSMB8 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(PSMB8 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PSMB8 antibody detected the PSMB8 protein (arrow).)
WB (Western Blot)
(PSMB8 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PSMB8 antibody detected the PSMB8 protein (arrow).)
Related Product Information for anti-PSMB8 antibody
The proteasome is a multicatalytic proteinase complex with
a highly ordered ring-shaped 20S core structure. The core structure
is composed of 4 rings of 28 non-identical subunits; 2 rings are
composed of 7 alpha subunits and 2 rings are composed of 7 beta
subunits. Proteasomes are distributed throughout eukaryotic cells
at a high concentration and cleave peptides in an
ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
essential function of a modified proteasome, the immunoproteasome,
is the processing of class I MHC peptides. This gene encodes a
member of the proteasome B-type family, also known as the T1B
family, that is a 20S core beta subunit. This gene is located in
the class II region of the MHC (major histocompatibility complex).
Expression of this gene is induced by gamma interferon and this
gene product replaces catalytic subunit 3 (proteasome beta 5
subunit) in the immunoproteasome. Proteolytic processing is
required to generate a mature subunit. Two alternative transcripts
encoding two isoforms have been identified; both isoforms are
processed to yield the same mature subunit.
a highly ordered ring-shaped 20S core structure. The core structure
is composed of 4 rings of 28 non-identical subunits; 2 rings are
composed of 7 alpha subunits and 2 rings are composed of 7 beta
subunits. Proteasomes are distributed throughout eukaryotic cells
at a high concentration and cleave peptides in an
ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
essential function of a modified proteasome, the immunoproteasome,
is the processing of class I MHC peptides. This gene encodes a
member of the proteasome B-type family, also known as the T1B
family, that is a 20S core beta subunit. This gene is located in
the class II region of the MHC (major histocompatibility complex).
Expression of this gene is induced by gamma interferon and this
gene product replaces catalytic subunit 3 (proteasome beta 5
subunit) in the immunoproteasome. Proteolytic processing is
required to generate a mature subunit. Two alternative transcripts
encoding two isoforms have been identified; both isoforms are
processed to yield the same mature subunit.
Product Categories/Family for anti-PSMB8 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Bravo, M.J., et al. Hum. Immunol. 71(7):708-711(2010)
Shi, C., et al. Epidemiol. Infect., 1-9 (2010) In press :
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Ustrell, V., et al. FEBS Lett. 376(3):155-158(1995)
Bravo, M.J., et al. Hum. Immunol. 71(7):708-711(2010)
Shi, C., et al. Epidemiol. Infect., 1-9 (2010) In press :
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Ustrell, V., et al. FEBS Lett. 376(3):155-158(1995)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30354
NCBI Official Full Name
proteasome subunit beta type-8 isoform E1 proprotein
NCBI Official Synonym Full Names
proteasome (prosome, macropain) subunit, beta type, 8
NCBI Official Symbol
PSMB8
NCBI Official Synonym Symbols
JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E
NCBI Protein Information
proteasome subunit beta type-8
UniProt Protein Name
Proteasome subunit beta type-8
UniProt Gene Name
PSMB8
UniProt Synonym Gene Names
LMP7; PSMB5i; RING10; Y2
UniProt Entry Name
PSB8_HUMAN
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Product Notes
The PSMB8 psmb8 (Catalog #AAA289566) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 8-36. The PSMB8 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMB8 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PSMB8 psmb8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMB8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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