IF (Immunofluorescence)
(Figure 2. IF analysis of PSMC2 using anti-PSMC2 antibody (AAA127577).PSMC2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PSMC2 Antibody (AAA127577) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Rabbit PSMC2 Polyclonal Antibody | anti-PSMC2 antibody
Anti-PSMC2 Antibody Picoband
Gene Names
PSMC2; S7; MSS1; Nbla10058
Reactivity
Human, Monkey
Applications
ELISA, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
PSMC2, Antibody; Anti-PSMC2 Antibody Picoband; CD72 molecule; B-cell differentiation antigen CD72; Lyb-2; CD72; anti-PSMC2 antibody
Host
Rabbit
Reactivity
Human, Monkey
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Pre-B-cells and B-cells but not terminally differentiated plasma cells.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-PSMC2 antibody
ELISA, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
E coli-derived human PSMC2 recombinant protein (Position: K34-N433).
Subcellular Localization
Membrane, Single-pass type II membrane protein
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Plays a role in B-cell proliferation and differentiation.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
IF (Immunofluorescence)
(Figure 2. IF analysis of PSMC2 using anti-PSMC2 antibody (AAA127577).PSMC2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PSMC2 Antibody (AAA127577) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(Figure 2. IF analysis of PSMC2 using anti-PSMC2 antibody (AAA127577).PSMC2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PSMC2 Antibody (AAA127577) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot)
(Figure 1. Western blot analysis of PSMC2 using anti-PSMC2 antibody (AAA127577).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MOLT-4 whole cell lysates,Lane 2: human T-47D whole cell lysates,Lane 3: monkey COS-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC2 antigen affinity purified polyclonal antibody (#AAA127577) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PSMC2 at approximately 49 kDa. The expected band size for PSMC2 is at 49 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of PSMC2 using anti-PSMC2 antibody (AAA127577).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MOLT-4 whole cell lysates,Lane 2: human T-47D whole cell lysates,Lane 3: monkey COS-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC2 antigen affinity purified polyclonal antibody (#AAA127577) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PSMC2 at approximately 49 kDa. The expected band size for PSMC2 is at 49 kDa.)
Related Product Information for anti-PSMC2 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-PSMC2 antibody
References
1. Dubiel, W., Ferrell, K., Rechsteiner, M. Peptide sequencing identifies MSS1, a modulator of HIV Tat-mediated transactivation, as subunit 7 of the 26 S protease. FEBS Lett. 323: 276-278, 1993. 2. Nelbock, P., Dillon, P. J., Perkins, A., Rosen, C. A. A cDNA for a protein that interacts with the human immunodeficiency virus Tat activator. Science 248: 1650-1653, 1990.3. Shibuya, H., Irie, K., Ninomiya-Tsuji, J., Goebl, M., Taniguchi, T., Matsumoto, K. New human gene encoding a positive modulator of HIV Tat-mediated transactivation. Nature 357: 700-702, 1992.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49kDa
NCBI Official Full Name
26S protease regulatory subunit 7 isoform 2
NCBI Official Synonym Full Names
proteasome (prosome, macropain) 26S subunit, ATPase, 2
NCBI Official Symbol
PSMC2
NCBI Official Synonym Symbols
S7; MSS1; Nbla10058
NCBI Protein Information
26S protease regulatory subunit 7; protease 26S subunit 7; proteasome 26S subunit ATPase 2; proteasome 26S subunit, ATPase, 2; putative protein product of Nbla10058; 26S proteasome AAA-ATPase subunit RPT1; mammalian suppressor of sgv-1 of yeast
UniProt Protein Name
26S protease regulatory subunit 7
UniProt Gene Name
PSMC2
UniProt Synonym Gene Names
MSS1
UniProt Entry Name
PRS7_HUMAN
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Product Notes
The PSMC2 psmc2 (Catalog #AAA127577) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSMC2 Antibody Picoband reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's PSMC2 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PSMC2 psmc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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