Rabbit anti-Human, Mouse PSMC2 Polyclonal Antibody | anti-PSMC2 antibody

Rabbit anti-PSMC2 Antibody, Affinity Purified

Cat. Num. AAA212298

• Gene Names: PSMC2; S7; MSS1; Nbla10058
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: PSMC2, Antibody; Rabbit anti-PSMC2 Antibody, Affinity Purified; 26S proteasome AAA-ATPase subunit RPT1; proteasome (prosome, macropain) 26S subunit, ATPase, 2; S7; putative protein product of Nbla10058; testis secretory sperm-binding protein Li 197a; Protein MSS1; Proteasome 26S subunit ATPase 2; protease 26S subunit 7; Nbla10058; MSS1; 26S proteasome regulatory subunit 7; 26S protease regulatory subunit 7; mammalian suppressor of sgv-1 of yeast; proteasome 26S subunit, ATPase 2; anti-PSMC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-PSMC2 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Predicted Reactivity: Rat, X. laevis, Bovine, Orangutan
• Immunogen: Between 175 and 225
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human PSMC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMC2 antibody AAA212298 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMC2 was also immunoprecipitated by rabbit anti-PSMC2 antibody A303-823A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)

IHC (Immunohiostchemistry)

(Detection of human PSMC2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PSMC2 (Cat. No. AAA212298 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of mouse PSMC2 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PSMC2 (Cat. No. AAA212298 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

Related Product Information for anti-PSMC2 antibody

Proteasome (prosome, macropain) 26S subunit, ATPase2 (PSMC2) is a subunit of the 26S proteasome, a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMC2 is one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors, so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. [taken from NCBI Entrez Gene (Gene ID: 5701)].

Product Categories/Family for anti-PSMC2 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 325053650
• NCBI GeneID: 5701
• NCBI Accession #: NP_002794.1
• NCBI GenBank Nucleotide #: NM_002803
• UniProt Accession #: P35998; Q3LIA5; Q9UDI3; A4D0Q1; B7Z5E2
• Molecular Weight: 33,274 Da
• NCBI Official Full Name: Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 2 (PSMC2), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, ATPase, 2
• NCBI Official Symbol: PSMC2
• NCBI Official Synonym Symbols: S7; MSS1; Nbla10058
• NCBI Protein Information: 26S protease regulatory subunit 7; 26S proteasome AAA-ATPase subunit RPT1; mammalian suppressor of sgv-1 of yeast; protease 26S subunit 7; proteasome 26S subunit ATPase 2; proteasome 26S subunit, ATPase, 2; putative protein product of Nbla10058
• UniProt Protein Name: 26S protease regulatory subunit 7
• UniProt Gene Name: PSMC2
• UniProt Synonym Gene Names: MSS1
• UniProt Entry Name: PRS7_HUMAN