IF (Immunofluorescence)
(Immunofluorescence staining of PSMC6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PSMC6 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)
Rabbit anti-Human PSMC6 Polyclonal Antibody | anti-PSMC6 antibody
Anti-PSMC6 Antibody, Rabbit Polyclonal
Gene Names
PSMC6; P44; p42; SUG2; CADP44
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PSMC6, Antibody; Anti-PSMC6 Antibody, Rabbit Polyclonal; PSMC6 Antibody, Rabbit PAb, Antigen Affinity Purified; proteasome (prosome, macropain) 26S subunit, ATPase, 6; Anti-CADP44 Antibody; Anti-HEL-S-73 Antibody; Anti-p42 Antibody; Anti-P44 Antibody; Anti-SUG2 Antibody; anti-PSMC6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PSMC6
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PSMC6 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human PSMC6
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human PSMC6, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of PSMC6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PSMC6 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of PSMC6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PSMC6 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)
WB (Western Blot)
(Anti-PSMC6 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDa)
WB (Western Blot)
(Anti-PSMC6 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDa)
Related Product Information for anti-PSMC6 antibody
PSMC6 (Proteasome 26S Subunit, ATPase 6) is a Protein Coding gene. The PSMC6 gene, located on 14q22.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, and mouse, etc. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. PSMC6 is widely expressed in the esophagus, brain, and other tissues. Diseases associated with PSMC6 include Cystic Fibrosis and Pheochromocytoma. The related pathways of PSMC6 are Regulation of activated PAK-2p34 by proteasome-mediated degradation and Antigen processing-Cross presentation. 237 organisms have orthologs with the human gene PSMC6.
References
Wu P, et al. (2015) Advanced oxidation protein products decrease the expression of calcium transport channels in small intestinal epithelium via the p44/42 mapk signaling pathway. Eur J Cell Biol 94 (5): 190-203.Wang Q, et al. (2010) Interactions of sars coronavirus nucleocapsid protein with the host cell proteasome subunit p42. Virol J 7 99.Hastings R, et al. (1999) Activator complexes containing the proteasomal regulatory atpases s10b (sug2) and s6 (tbp1) in different tissues and organisms. Mol Biol Rep 26 (1-2): 35-38.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,173 Da
NCBI Official Full Name
26S protease regulatory subunit 10B
NCBI Official Synonym Full Names
proteasome (prosome, macropain) 26S subunit, ATPase, 6
NCBI Official Symbol
PSMC6
NCBI Official Synonym Symbols
P44; p42; SUG2; CADP44
NCBI Protein Information
26S protease regulatory subunit 10B; proteasome subunit p42; proteasome 26S subunit ATPase 6; conserved ATPase domain protein 44; 26S protease regulatory subunit S10B; 26S proteasome AAA-ATPase subunit RPT4
UniProt Protein Name
26S protease regulatory subunit 10B
UniProt Gene Name
PSMC6
UniProt Synonym Gene Names
SUG2
UniProt Entry Name
PRS10_HUMAN
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Product Notes
The PSMC6 psmc6 (Catalog #AAA255191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSMC6 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMC6 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PSMC6 psmc6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMC6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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