WB (Western Blot)
(Detection of human and mouse PSMC6 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMC6 antibody AAA212300 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMC6 was also immunoprecipitated by rabbit anti-PSMC6 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
Rabbit anti-Human, Mouse PSMC6 Polyclonal Antibody | anti-PSMC6 antibody
Rabbit anti-PSMC6 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PSMC6, Antibody; Rabbit anti-PSMC6 Antibody, Affinity Purified; 26S proteasome AAA-ATPase subunit RPT4; SUG2; proteasome subunit p42; Proteasome 26S subunit ATPase 6; proteasome (prosome, macropain) 26S subunit, ATPase, 6; p42; 26S proteasome regulatory subunit 10B; 26S protease regulatory subunit 10B; proteasome 26S subunit, ATPase 6; anti-PSMC6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PSMC6 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 339 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse PSMC6 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMC6 antibody AAA212300 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMC6 was also immunoprecipitated by rabbit anti-PSMC6 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human and mouse PSMC6 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMC6 antibody AAA212300 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMC6 was also immunoprecipitated by rabbit anti-PSMC6 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human PSMC6 by immunohistochemistry. Samples: FFPE serial sections of human lung carcinoma. Antibody: Affinity purified rabbit anti-PSMC6 (Cat. No. Lot1, left image and Cat. No. AAA212300 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human PSMC6 by immunohistochemistry. Samples: FFPE serial sections of human lung carcinoma. Antibody: Affinity purified rabbit anti-PSMC6 (Cat. No. Lot1, left image and Cat. No. AAA212300 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
Related Product Information for anti-PSMC6 antibody
Proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6) is a subunit of the 26S proteasome, a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMC6 is one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity [taken from NCBI Entrez Gene (Gene ID: 5706)].
Product Categories/Family for anti-PSMC6 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PSMC6 psmc6 (Catalog #AAA212300) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PSMC6 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PSMC6 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PSMC6 psmc6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMC6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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