WB (Western Blot)
(Detection of human PSMD4 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMD4 antibody AAA212323 (lot AAA212323-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human PSMD4 Polyclonal Antibody | anti-PSMD4 antibody
Rabbit anti-PSMD4 Antibody, Affinity Purified
Gene Names
PSMD4; AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PSMD4, Antibody; Rabbit anti-PSMD4 Antibody, Affinity Purified; multiubiquitin chain-binding protein; S5a/antisecretory factor protein; S5A; Rpn10; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; MCB1; ASF; angiocidin; 26S proteasome non-ATPase regulatory subunit 4; AF-1; AF; 26S proteasome regulatory subunit S5A; 26S proteasome regulatory subunit RPN10; antisecretory factor 1; pUB-R5; proteasome 26S subunit, non-ATPase 4; anti-PSMD4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PSMD4 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 327 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PSMD4 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMD4 antibody AAA212323 (lot AAA212323-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human PSMD4 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMD4 antibody AAA212323 (lot AAA212323-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PSMD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSMD4 antibody AAA212323 (lot AAA212323-1) used for IP at 6 ug/mg lysate. PSMD4 was also immunoprecipitated by rabbit anti-PSMD4 antibody For blotting immunoprecipitated PSMD4, AAA212323 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human PSMD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSMD4 antibody AAA212323 (lot AAA212323-1) used for IP at 6 ug/mg lysate. PSMD4 was also immunoprecipitated by rabbit anti-PSMD4 antibody For blotting immunoprecipitated PSMD4, AAA212323 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human PSMD4 by immunohistochemistry. Samples: FFPE sections of human lung carcinoma. Antibody: Affinity purified rabbit anti-PSMD4 Cat. No. Lot1 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. AAA212323 Lot1 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PSMD4 by immunohistochemistry. Samples: FFPE sections of human lung carcinoma. Antibody: Affinity purified rabbit anti-PSMD4 Cat. No. Lot1 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. AAA212323 Lot1 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB)
Related Product Information for anti-PSMD4 antibody
PSMD4 [proteasome (prosome, macropain) 26S subunit, non-ATPase, 4] is a non-ATPase subunit of the 19S regulator lid. The 19S regulator and 20S core complexes make up the 26S proteasome which is a multicatalytic proteinase complex with a highly ordered structure. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides [taken from NCBI Entrez Gene (Gene ID: 5710)].
Product Categories/Family for anti-PSMD4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,612 Da
NCBI Official Full Name
26S proteasome non-ATPase regulatory subunit 4 isoform 2
NCBI Official Synonym Full Names
proteasome 26S subunit, non-ATPase 4
NCBI Official Symbol
PSMD4
NCBI Official Synonym Symbols
AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
NCBI Protein Information
26S proteasome non-ATPase regulatory subunit 4
UniProt Protein Name
26S proteasome non-ATPase regulatory subunit 4
UniProt Gene Name
PSMD4
UniProt Synonym Gene Names
MCB1; AF; ASF
UniProt Entry Name
PSMD4_HUMAN
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Product Notes
The PSMD4 psmd4 (Catalog #AAA212323) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PSMD4 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMD4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PSMD4 psmd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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