Application Data
(Normalized signal intensity of PSMD4 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMD4 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.)
Rabbit anti-Human PSMD4 Polyclonal Antibody | anti-PSMD4 antibody
PSMD4 antibody
Gene Names
PSMD4; AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
Reactivity
Human
Applications
Western Blot
Purity
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Synonyms
PSMD4, Antibody; PSMD4 antibody; RABBIT ANTI PSMD4; anti-PSMD4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Form/Format
Purified IgG - liquid
Phosphate buffered saline
0.09% Sodium Azide, <50% Glycerol
Phosphate buffered saline
0.09% Sodium Azide, <50% Glycerol
Concentration
IgG concentration 0.5mg/ml (varies by lot)
Applicable Applications for anti-PSMD4 antibody
WB (Western Blot)
Species Cross-Reactivity
Rat:Yes
Immunogen
Recombinant protein of human PSMD4
Preparation and Storage
Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles
12 months from date of despatch.
12 months from date of despatch.
Application Data
(Normalized signal intensity of PSMD4 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMD4 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.)
Application Data
(Normalized signal intensity of PSMD4 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMD4 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.)
WB (Western Blot)
(Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMD4 (1 & 2) or a scrambled siRNA (-) probed with Rabbit anti PSMD4 antibody (AAA50467) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/5,000 1705046) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc M)
WB (Western Blot)
(Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMD4 (1 & 2) or a scrambled siRNA (-) probed with Rabbit anti PSMD4 antibody (AAA50467) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/5,000 1705046) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc M)
WB (Western Blot)
(Western blot analysis of whole cell lysates probed with PSMD4 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to PSMD4 (molecular weight 50 kDa))
WB (Western Blot)
(Western blot analysis of whole cell lysates probed with PSMD4 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to PSMD4 (molecular weight 50 kDa))
Related Product Information for anti-PSMD4 antibody
Rabbit anti Human PSMD4 antibody recognizes the PSMD4, also known as 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit S5A, S5a/antisecretory factor protein, angiocidin and multiubiquitin chain-binding protein.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21 (provided by RefSeq, Jul 2008).
Mouse anti Human PSMD4 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21 (provided by RefSeq, Jul 2008).
Mouse anti Human PSMD4 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
377
NCBI Official Full Name
26S proteasome non-ATPase regulatory subunit 4
NCBI Official Synonym Full Names
proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
NCBI Official Symbol
PSMD4
NCBI Official Synonym Symbols
AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
NCBI Protein Information
26S proteasome non-ATPase regulatory subunit 4; angiocidin; antisecretory factor 1; S5a/antisecretory factor protein; multiubiquitin chain-binding protein; 26S proteasome regulatory subunit S5A
UniProt Protein Name
26S proteasome non-ATPase regulatory subunit 4
UniProt Gene Name
PSMD4
UniProt Synonym Gene Names
MCB1; AF; ASF
UniProt Entry Name
PSMD4_HUMAN
Similar Products
Product Notes
The PSMD4 psmd4 (Catalog #AAA50467) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSMD4 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMD4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PSMD4 psmd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.