WB (Western Blot)
(Detection of human PSMD5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PSMD5 antibody AAA213046 (lot AAA213046-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human PSMD5/26S Proteasome Subunit S5B Polyclonal Antibody | anti-PSMD5 antibody
Rabbit anti-PSMD5/26S Proteasome Subunit S5B Antibody, Affinity Purified
Gene Names
PSMD5; S5B
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PSMD5/26S Proteasome Subunit S5B, Antibody; Rabbit anti-PSMD5/26S Proteasome Subunit S5B Antibody, Affinity Purified; 26S protease subunit S5 basic; S5B; proteasome (prosome, macropain) 26S subunit, non-ATPase, 5; 26S proteasome non-ATPase regulatory subunit 5; 26S proteasome subunit S5B; proteasome 26S subunit, non-ATPase 5; anti-PSMD5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PSMD5 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 325 and 375
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PSMD5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PSMD5 antibody AAA213046 (lot AAA213046-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PSMD5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PSMD5 antibody AAA213046 (lot AAA213046-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PSMD5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PSMD5 antibody AAA213046 (lot AAA213046-1) used for IP at 6 ug per reaction. PSMD5 was also immunoprecipitated by rabbit anti-PSMD5 antibody BL19749. For blotting immunoprecipitated PSMD5, AAA213046 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PSMD5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PSMD5 antibody AAA213046 (lot AAA213046-1) used for IP at 6 ug per reaction. PSMD5 was also immunoprecipitated by rabbit anti-PSMD5 antibody BL19749. For blotting immunoprecipitated PSMD5, AAA213046 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-PSMD5 antibody
PSMD5/26S Proteasome Subunit S5B acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16401].
Product Categories/Family for anti-PSMD5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,312 Da
NCBI Official Full Name
26S proteasome non-ATPase regulatory subunit 5 isoform 1
NCBI Official Synonym Full Names
proteasome 26S subunit, non-ATPase 5
NCBI Official Symbol
PSMD5
NCBI Official Synonym Symbols
S5B
NCBI Protein Information
26S proteasome non-ATPase regulatory subunit 5
UniProt Protein Name
26S proteasome non-ATPase regulatory subunit 5
UniProt Gene Name
PSMD5
UniProt Synonym Gene Names
KIAA0072
UniProt Entry Name
PSMD5_HUMAN
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Product Notes
The PSMD5 psmd5 (Catalog #AAA213046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PSMD5/26S Proteasome Subunit S5B Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMD5/26S Proteasome Subunit S5B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PSMD5 psmd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMD5/26S Proteasome Subunit S5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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