Rabbit anti-Human PSME3 Polyclonal Antibody | anti-PSME3 antibody

Anti-PSME3 Antibody, Rabbit Polyclonal

Cat. Num. AAA255789

• Gene Names: PSME3; Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: PSME3, Antibody; Anti-PSME3 Antibody, Rabbit Polyclonal; PSME3 Antibody, Rabbit PAb, Antigen Affinity Purified; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki); Anti-HEL-S-283 Antibody; Anti-Ki Antibody; Anti-PA28-gamma Antibody; Anti-PA28G Antibody; Anti-PA28gamma Antibody; Anti-REG-GAMMA Antibody; anti-PSME3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PSME3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PSME3 antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human PSME3 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human PSME3 (rh PSME3). PSME3 specific IgG was purified by Human PSME3 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(PSME3 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg A-431 Whole Cell LysateLane C:0.5 mg MCF7 Whole Cell LysateLane D:0.5 mg HepG2 Whole Cell Lysate4uL anti-PSME3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSME3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :34 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of PSME3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PSME3 polyclonal antibody (dilution ratio 1:300) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.)

IHC (Immunohistochemisry)

(Immunochemical staining of human PSME3 in human colon carcinoma with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human PSME3 in human breast carcinoma with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-PSME3 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:30 kDa)

Related Product Information for anti-PSME3 antibody

PSME3, also known as PA28-gamma, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 2S core and a 19S regulator. The 2S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 1 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME3 gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring.

References

Albertsen HM. et al., 1994, Nat Genet. 7 (4): 472-9. Zhang Zhuo. et al., 2008, EMBO J. 27 (6): 852-64. Coux O. et al., 1996, Annu Rev Biochem. 65: 801-47.

NCBI and Uniprot Product Information

• NCBI GI #: 388556516
• NCBI GeneID: 10197
• NCBI Accession #: NP_001253974.1
• NCBI GenBank Nucleotide #: NM_001267045.1
• UniProt Accession #: P61289; O35563; P97373; Q12920; Q13172; Q9BQD9; A8K9A3
• Molecular Weight: 29,506 Da
• NCBI Official Full Name: proteasome activator complex subunit 3 isoform 3
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
• NCBI Official Symbol: PSME3
• NCBI Official Synonym Symbols: Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
• NCBI Protein Information: proteasome activator complex subunit 3; Ki antigen; REG gamma-3; PA28 gamma variant 5; Ki nuclear autoantigen; proteasome activator 28-gamma; 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; epididymis secretory protein Li 283; pr
• UniProt Protein Name: Proteasome activator complex subunit 3
• UniProt Gene Name: PSME3
• UniProt Synonym Gene Names: REG-gamma; PA28g; PA28gamma
• UniProt Entry Name: PSME3_HUMAN