WB (Western Blot)
(Detection of human PUF60 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody AAA212031 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
Rabbit anti-Human, Mouse PUF60 Polyclonal Antibody | anti-PUF60 antibody
Rabbit anti-PUF60 Antibody, Affinity Purified
Gene Names
PUF60; FIR; VRJS; RoBPI; SIAHBP1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PUF60, Antibody; Rabbit anti-PUF60 Antibody, Affinity Purified; pyrimidine tract binding splicing factor; Siah-BP1; SIAHBP1; Siah-binding protein 1; Siah binding protein 1; RoBPI; RoBP1; 60 kDa poly(U)-binding-splicing factor; Ro ribonucleoprotein-binding protein 1; poly(U)-binding-splicing factor PUF60; poly(U) binding splicing factor 60KDa; FUSE-binding protein-interacting repressor; FIR; FBP-interacting repressor; FBP interacting repressor; VRJS; Ro-binding protein 1; Poly(U)-binding-splicing factor PUF60; poly(U) binding splicing factor 60; anti-PUF60 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PUF60 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 400 and 450
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PUF60 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody AAA212031 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human PUF60 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody AAA212031 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human and mouse PUF60 by immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PUF60 (Cat. No. AAA212031) used at a dilution of 1:200 (1ug/ml) Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human and mouse PUF60 by immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PUF60 (Cat. No. AAA212031) used at a dilution of 1:200 (1ug/ml) Detection: DAB)
Related Product Information for anti-PUF60 antibody
Poly-U binding splicing factor 60KDa (PUF60) is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. PUF60 is implicated in the xeroderma pigmentosum disorder [taken from NCBI Entrez Gene (Gene ID: 22827)].
Product Categories/Family for anti-PUF60 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,399 Da
NCBI Official Full Name
poly(U)-binding-splicing factor PUF60 isoform a
NCBI Official Synonym Full Names
poly(U) binding splicing factor 60KDa
NCBI Official Symbol
PUF60
NCBI Official Synonym Symbols
FIR; VRJS; RoBPI; SIAHBP1
NCBI Protein Information
poly(U)-binding-splicing factor PUF60
UniProt Protein Name
Poly(U)-binding-splicing factor PUF60
UniProt Gene Name
PUF60
UniProt Synonym Gene Names
FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1
UniProt Entry Name
PUF60_HUMAN
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Product Notes
The PUF60 puf60 (Catalog #AAA212031) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PUF60 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PUF60 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PUF60 puf60 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PUF60, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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