WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:Hep G2; B:MOLT4; C: Raji) using a 7.5% SDS PAGE gel and PUF60 antibody at a dilution of 1:500)
Rabbit PUF60 Polyclonal Antibody | anti-PUF60 antibody
PUF60 Antibody
Gene Names
PUF60; FIR; VRJS; RoBPI; SIAHBP1
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Antigen-Affinity Chromatography
Synonyms
PUF60, Antibody; PUF60 Antibody; Affinity Purified Rabbit Polyclonal PUF60 Antibody; Polyclonal PUF60 antibody; Anti-PUF60 antibody; ro-binding protein 1 antibody; SIAHBP1 antibody; PUF-60 antibody; poly-U binding splicing factor PUF60 antibody; siah binding protein 1 antibody; FBP interacting repressor antibody; poly(U)-binding-splicing factor PUF60 antibody; Ro ribonucleoprotein-binding protein 1 antibody; roBP1 antibody; PUF60 antibody; PUF 60; RoBPI antibody; PUF60; siah-BP1 antibody; FIR antibody; FLJ31379 antibody; Ro ribonucleoprotein binding protein 1 antibody; PUF 60 antibody; PUF-60; anti-PUF60 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.81 mg/ml (varies by lot)
Sequence Length
559
Applicable Applications for anti-PUF60 antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
PUF60 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 346 and 523 of PUF60
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:Hep G2; B:MOLT4; C: Raji) using a 7.5% SDS PAGE gel and PUF60 antibody at a dilution of 1:500)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:Hep G2; B:MOLT4; C: Raji) using a 7.5% SDS PAGE gel and PUF60 antibody at a dilution of 1:500)
IHC (Immunohiostchemistry)
(Immunohistochemical staining of paraffin-embedded oral tissue using PUF60 antibody at a dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemical staining of paraffin-embedded oral tissue using PUF60 antibody at a dilution of 1:100)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using PUF60 antibody at 1:500 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using PUF60 antibody at 1:500 dilution.)
Related Product Information for anti-PUF60 antibody
The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder.
Product Categories/Family for anti-PUF60 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58 kDa
NCBI Official Full Name
poly(U)-binding-splicing factor PUF60 isoform a
NCBI Official Synonym Full Names
poly(U) binding splicing factor 60
NCBI Official Symbol
PUF60
NCBI Official Synonym Symbols
FIR; VRJS; RoBPI; SIAHBP1
NCBI Protein Information
poly(U)-binding-splicing factor PUF60
UniProt Protein Name
Poly(U)-binding-splicing factor PUF60
UniProt Gene Name
PUF60
UniProt Synonym Gene Names
FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1
UniProt Entry Name
PUF60_HUMAN
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Product Notes
The PUF60 puf60 (Catalog #AAA225141) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PUF60 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the PUF60 puf60 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PUF60, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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