ICC (Immunocytochemistry)
(Immunocytochemistry of PUMA in K562 cells with PUMA antibody at 1 ug/ml.)
Rabbit anti-Human, Mouse PUMA Polyclonal Antibody | anti-PUMA antibody
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column
Synonyms
PUMA, Antibody; PUMA Antibody; bbc3; anti-PUMA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
193
Applicable Applications for anti-PUMA antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen Type
Peptide
Immunogen Description
Raised against a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human PUMA-a. This sequence is identical between a and b forms of the PUMA proteins.
Target Name
PUMA
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ICC (Immunocytochemistry)
(Immunocytochemistry of PUMA in K562 cells with PUMA antibody at 1 ug/ml.)
ICC (Immunocytochemistry)
(Immunocytochemistry of PUMA in K562 cells with PUMA antibody at 1 ug/ml.)
WB (Western Blot)
(Western blot analysis of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lysates with PUMA antibody at 2 ug/ml)
WB (Western Blot)
(Western blot analysis of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lysates with PUMA antibody at 2 ug/ml)
Related Product Information for anti-PUMA antibody
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Product Categories/Family for anti-PUMA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,326 Da
NCBI Official Full Name
bcl-2-binding component 3 isoform 4
NCBI Official Synonym Full Names
BCL2 binding component 3
NCBI Official Symbol
BBC3
NCBI Official Synonym Symbols
JFY1; PUMA; JFY-1
NCBI Protein Information
bcl-2-binding component 3
UniProt Protein Name
Bcl-2-binding component 3
UniProt Gene Name
BBC3
UniProt Synonym Gene Names
PUMA
UniProt Entry Name
BBC3_HUMAN
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Product Notes
The PUMA bbc3 (Catalog #AAA301059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PUMA Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PUMA can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PUMA bbc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PUMA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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