Rabbit anti-Human, Mouse PUMA Polyclonal Antibody | anti-PUMA antibody

Rabbit Polyclonal to Human PUMA

Cat. Num. AAA50780

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: PUMA, Antibody; Rabbit Polyclonal to Human PUMA; Anti-PUMA Antibody (C-Terminus) IHC-plus; BBC3; Bcl-2-binding component 3; BCL2 binding component 3; JFY-1; JFY1; PUMA; Human PUMA; anti-PUMA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human PUMA-a (GenBank Accession number Q9BXH1) This sequence is identical between a and b forms of the PUMA proteins
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 193

• Applicable Applications for anti-PUMA antibody: WB (Western Blot), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Description: A synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human PUMA-a (GenBank Accession number Q9BXH1) This sequence is identical between a and b forms of the PUMA proteins.
• Immunogen Type: Synthetic peptide
• Immunogen: PUMA antibody was raised against a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human PUMA-a (GenBank Accession number Q9BXH1) This sequence is identical between a and b forms of the PUMA proteins.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

WB (Western Blot)

(Western blot of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lysates with PUMA antibody at 2 ug/ml)

ICC (Immunocytochemistry)

(Immunocytochemistry of PUMA in K562 cells with PUMA antibody at 1 ug/ml.)

IF (Immunofluorescence)

(Immunofluorescence of PUMA in K562 cells with PUMA antibody at 2 ug/ml.)

IHC (Immunohiostchemistry)

(Anti-BBC3 / PUMA antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

IHC (Immunohistochemistry)

(Anti-BBC3 / PUMA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-PUMA antibody

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53.

Product Categories/Family for anti-PUMA antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 15193488
• NCBI GeneID: 27113
• NCBI Accession #: NP_055232.1
• NCBI GenBank Nucleotide #: NM_014417.4
• UniProt Accession #: Q9BXH1; O00171; Q96PG9; B9EGI3; Q96PG8
• Molecular Weight: 10,326 Da
• NCBI Official Full Name: bcl-2-binding component 3 isoform 4
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
• UniProt Protein Name: Bcl-2-binding component 3
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3_HUMAN