DB (Dot Blot)
(The 2'-O-methyluridine(Um) Rabbit pAb are tested in Dot Blot against 2'-O-methyluridine(Um) and unmodified uridine.Um-3?5'Biotin-UGACAACUACAGAC(Um)3'Um-4?5'Biotin-UGACAACUACAGACU)
Rabbit Puromycin Polyclonal Antibody | anti-Puromycin antibody
Puromycin Rabbit pAb
Applications
Dot Blot, ELISA
Purity
Affinity purification
Synonyms
Puromycin, Antibody; Puromycin Rabbit pAb; Um; 2'-O-methyluridine; 2'-O-methyluridine(Um); anti-Puromycin antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.01% thimerosal, 50% glycerol, pH 7.3.
Applicable Applications for anti-Puromycin antibody
DB (Dot Blot), ELISA
Cross-Reactivity
Species independent
Immunogen
Chemical compounds corresponding to 2'-O-methyluridine(Um).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
DB (Dot Blot)
(The 2'-O-methyluridine(Um) Rabbit pAb are tested in Dot Blot against 2'-O-methyluridine(Um) and unmodified uridine.Um-3?5'Biotin-UGACAACUACAGAC(Um)3'Um-4?5'Biotin-UGACAACUACAGACU)
DB (Dot Blot)
(The 2'-O-methyluridine(Um) Rabbit pAb are tested in Dot Blot against 2'-O-methyluridine(Um) and unmodified uridine.Um-3?5'Biotin-UGACAACUACAGAC(Um)3'Um-4?5'Biotin-UGACAACUACAGACU)
WB (Western Blot)
(Western blot analysis of RAW264.7, using Puromycin antibody at 1:2210 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of RAW264.7, using Puromycin antibody at 1:2210 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of 293F, using Puromycin antibody at 1:2210 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of 293F, using Puromycin antibody at 1:2210 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Related Product Information for anti-Puromycin antibody
RNA methylation plays a significant regulatory role in various of physiological activities and it has gradually become a hotspot of epigenetics in the past decade. 2'-O-methyladenosine (Am), 2'-O-methylguanosine (Gm),2'-O-methylcytidine (Cm),2'-O-methyluridine (Um),N 6-methyladenosine (m6A), N 1-methylguanosine (m1G),5-methylcytidine (m5C),and 5-methyluridine (m5U) are representative 2'-O-methylation and base-methylation modified epigenetic marks of RNA. 2'-O-methyltransferase is a modified nucleoside that is produced in tRNAs by the action of tRNA guanosine-2'-O-methyltransferase, using S-adenosyl-L-methionine as a substrate. Through its interaction with other modified nucleosides, 2'-O-methylguanosine is thought to stabilize the structure of the tRNA.
Product Categories/Family for anti-Puromycin antibody
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Product Notes
The Puromycin (Catalog #AAA282191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Puromycin can be used in a range of immunoassay formats including, but not limited to, DB (Dot Blot), ELISA. Researchers should empirically determine the suitability of the Puromycin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Puromycin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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