Rabbit PYCARD Polyclonal Antibody | anti-PYCARD antibody

Phospho-PYCARD (Ser195) Antibody

Cat. Num. AAA31280

• Gene Names: PYCARD; ASC; TMS; TMS1; CARD5; TMS-1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: PYCARD, Antibody; Phospho-PYCARD (Ser195) Antibody; Apoptosis associated speck like protein containing a CARD; Apoptosis-associated speck-like protein containing a CARD; ASC; ASC_HUMAN; CARD 5; CARD5; Caspase recruitment domain containing protein 5; Caspase recruitment domain protein 5; Caspase recruitment domain-containing protein 5; hASC; MGC10332; PYCARD; PYD and CARD domain containing; PYD and CARD domain containing protein; PYD and CARD domain-containing protein; Target of methylation induced silencing 1; Target of methylation-induced silencing 1; TMS 1; TMS; TMS1; anti-PYCARD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PYCARD antibody: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: IF/ICC 1:100-1:500; IHC 1:50-1:200; *The optimal dilutions should be determined by the end user.
• Immunogen: A synthesized peptide derived from human TMS1/ASC around the phosphorylation site of Ser195.
• Preparation and Storage: Store at -20 °C. Stable for 12 months from date of receipt.

IF (Immunofluorescence)

(AAA31280 staining A549 cells(H2O2 treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#AAA31280) and mouse anti-beta tubulin Ab(#) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)

IHC (Immunohistchemistry)

(At 1/100 staining Human prostate cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(Staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 18203507
• NCBI GeneID: 29108
• UniProt Accession #: Q9ULZ3; Q96D12; Q9BSZ5; Q9HBD0; Q9NXJ8
• NCBI Official Full Name: Apoptosis-associated speck-like protein containing a CARD
• NCBI Official Synonym Full Names: PYD and CARD domain containing
• NCBI Official Symbol: PYCARD
• NCBI Official Synonym Symbols: ASC; TMS; TMS1; CARD5; TMS-1
• NCBI Protein Information: apoptosis-associated speck-like protein containing a CARD; target of methylation-induced silencing 1; caspase recruitment domain-containing protein 5
• UniProt Protein Name: Apoptosis-associated speck-like protein containing a CARD
• UniProt Gene Name: PYCARD
• UniProt Synonym Gene Names: ASC; CARD5; TMS1; hASC
• UniProt Entry Name: ASC_HUMAN