Rabbit PYCARD Polyclonal Antibody | anti-PYCARD antibody

PYCARD Polyclonal Antibody

Cat. Num. AAA179558

• Gene Names: Pycard; Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity purification
• Synonyms: PYCARD, Antibody; PYCARD Polyclonal Antibody; A; Asc; TMS-; TNS1; masc; CARD5; TMS-1; 9130417A21Rik; anti-PYCARD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-PYCARD antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant fusion protein of mouse PYCARD
• Preparation and Storage: Storage: -20 degree C/One year. Avoid freeze/thaw cycles.

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat heart using PYCARD Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse kidney using PYCARD Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded human liver cancer using PYCARD Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines using PYCARD Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines using PYCARD Polyclonal Antibody at 1:1000 dilution.)

Related Product Information for anti-PYCARD antibody

Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions.

Product Categories/Family for anti-PYCARD antibody

Antibodies; Primary Antibodies; Polyclonal Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 165377185
• NCBI GeneID: 66824
• NCBI Accession #: NP_075747.3
• NCBI GenBank Nucleotide #: NM_023258.4
• UniProt Accession #: Q9EPB4; Q9D2W9
• Molecular Weight: 21,459 Da
• NCBI Official Full Name: apoptosis-associated speck-like protein containing a CARD
• NCBI Official Synonym Full Names: PYD and CARD domain containing
• NCBI Official Symbol: Pycard
• NCBI Official Synonym Symbols: Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik
• NCBI Protein Information: apoptosis-associated speck-like protein containing a CARD
• UniProt Protein Name: Apoptosis-associated speck-like protein containing a CARD
• UniProt Gene Name: Pycard
• UniProt Synonym Gene Names: Asc; mASC
• UniProt Entry Name: ASC_MOUSE