IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit PYGM Polyclonal Antibody | anti-PYGM antibody
PYGM Antibody (C-term)
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PYGM, Antibody; PYGM Antibody (C-term); Glycogen phosphorylase; muscle form; Myophosphorylase; PYGM; anti-PYGM antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PYGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from the C-terminal region of human PYGM.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
698-727
Sequence Length
754
Applicable Applications for anti-PYGM antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of PYGM (arrow) using rabbit polyclonal PYGM Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of PYGM (arrow) using rabbit polyclonal PYGM Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-PYGM antibody
PYGM catalyzes and regulates the breakdown of glycogen to glucose-1-phosphate. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5), also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.
Product Categories/Family for anti-PYGM antibody
References
Tsoi, S.C., et al., J. Soc. Gynecol. Investig. 10(8):496-502 (2003).
Bruno, C., et al., Neuromuscul. Disord. 12(5):498-500 (2002).
Hadjigeorgiou, G.M., et al., Neuromuscul. Disord. 12(9):824-827 (2002).
Deschauer, M., et al., Mol. Genet. Metab. 74(4):489-491 (2001).
Kubisch, C., et al., Hum. Mutat. 12(1):27-32 (1998).
Bruno, C., et al., Neuromuscul. Disord. 12(5):498-500 (2002).
Hadjigeorgiou, G.M., et al., Neuromuscul. Disord. 12(9):824-827 (2002).
Deschauer, M., et al., Mol. Genet. Metab. 74(4):489-491 (2001).
Kubisch, C., et al., Hum. Mutat. 12(1):27-32 (1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97092
NCBI Official Full Name
glycogen phosphorylase, muscle form isoform 2
NCBI Official Synonym Full Names
phosphorylase, glycogen, muscle
NCBI Official Symbol
PYGM
NCBI Protein Information
glycogen phosphorylase, muscle form
UniProt Protein Name
Glycogen phosphorylase, muscle form
UniProt Gene Name
PYGM
UniProt Entry Name
PYGM_HUMAN
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Product Notes
The PYGM pygm (Catalog #AAA285785) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 698-727. The PYGM Antibody (C-term) reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's PYGM can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PYGM pygm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PYGM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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