<
IF (Immunofluorescence)
(Pyruvate Kinase antibody detects PKLR protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in - 20 deg C 100% MeOH for 5 min. Green: PKLR protein stained by Pyruvate Kinase (liver/RBC) antibody diluted at 1:500. Blue: Hoechst 33343 staining.)
Rabbit Pyruvate Kinase Polyclonal Antibody | anti-PK1 antibody
Pyruvate Kinase Antibody
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Antigen-Affinity Chromatography
Synonyms
Pyruvate Kinase, Antibody; Pyruvate Kinase Antibody; Affinity Purified Rabbit Polyclonal Pyruvate Kinase Antibody; Polyclonal Pyruvate Kinase antibody; Anti-Pyruvate Kinase antibody; PKL antibody; RPK antibody; PKR antibody; PKRL antibody; pyruvate kinase type L antibody; R-type/L-type pyruvate kinase antibody; PK1 antibody; red cell/liver pyruvate kinase antibody; PKLR antibody; anti-PK1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
2430
Applicable Applications for anti-PK1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Pyruvate Kinase antibody detects PKLR protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in - 20 deg C 100% MeOH for 5 min. Green: PKLR protein stained by Pyruvate Kinase (liver/RBC) antibody diluted at 1:500. Blue: Hoechst 33343 staining.)
IF (Immunofluorescence)
(Pyruvate Kinase antibody detects PKLR protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in - 20 deg C 100% MeOH for 5 min. Green: PKLR protein stained by Pyruvate Kinase (liver/RBC) antibody diluted at 1:500. Blue: Hoechst 33343 staining.)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: 293T; B: A431; C: HeLa; D: HepG2; E: A375) using a 7.5% SDS PAGE gel and Pyruvate Kinase antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: 293T; B: A431; C: HeLa; D: HepG2; E: A375) using a 7.5% SDS PAGE gel and Pyruvate Kinase antibody at a dilution of 1:1000)
IHC (Immunohiostchemistry)
(Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using Pyruvate Kinase antibody at a dilution of 1:500)
IHC (Immunohiostchemistry)
(Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using Pyruvate Kinase antibody at a dilution of 1:500)
WB (Western Blot)
(Western blot analysis of A. 20 ug Rat liver lysate/extract using a 7.5% SDS PAGE gel and Pyruvate Kinase antibody at a dilution of 1:10000)
WB (Western Blot)
(Western blot analysis of A. 20 ug Rat liver lysate/extract using a 7.5% SDS PAGE gel and Pyruvate Kinase antibody at a dilution of 1:10000)
Related Product Information for anti-PK1 antibody
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia.
Product Categories/Family for anti-PK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62 kDa
NCBI Official Full Name
pyruvate kinase
NCBI Official Symbol
PYK4a
NCBI Protein Information
pyruvate kinase
Similar Products
Product Notes
The PK1 (Catalog #AAA225542) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Pyruvate Kinase can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the PK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyruvate Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.