Rabbit RABGAP1 Polyclonal Antibody | anti-RABGAP1 antibody

RABGAP1 Antibody (N-term)

Cat. Num. AAA287164

• Gene Names: RABGAP1; GAPCENA; TBC1D11
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: RABGAP1, Antibody; RABGAP1 Antibody (N-term); Rab GTPase-activating protein 1; GAP and centrosome-associated protein; Rab6 GTPase-activating protein GAPCenA; RABGAP1; anti-RABGAP1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This RABGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human RABGAP1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 14-43
• Sequence Length: 265

• Applicable Applications for anti-RABGAP1 antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Function: May act as a GTPase-activating protein of RAB6A. May play a role in microtubule nucleation by centrosome. May participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition.
• Cellular Location: Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly cytosolic but also associated with the centrosome.
• Preparation and Storage: Maintain refrigerated at 2°C to 8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(RABGAP1 Antibody (N-term) (Cat. # AAA287164) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(RABGAP1 Antibody (N-term) (Cat. #AAA287164) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RABGAP1 antibody detected the RABGAP1 protein (arrow).)

Related Product Information for anti-RABGAP1 antibody

RABGAP1 may act as a GTPase-activating protein of RAB6A and play a role in microtubule nucleation by centrosome. It may participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition.

Product Categories/Family for anti-RABGAP1 antibody

Cell Biology; Signal transduction

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ishibashi, K., et al. Genes Cells 14(1):41-52(2009)
Echard, A., et al. Mol. Biol. Cell 11(11):3819-3833(2000)
Opdam, F.J., et al. J. Cell. Sci. 113 (PT 15), 2725-2735 (2000) :
Cuif, M.H., et al. EMBO J. 18(7):1772-1782(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 164519084
• NCBI GeneID: 23637
• NCBI Accession #: NP_036329.3
• NCBI GenBank Nucleotide #: NM_012197.3
• UniProt Accession #: Q9Y3P9; Q05CW2; Q6ZMY1; Q9HA28; Q9P0E2; Q9UG67; B9A6L2
• Molecular Weight: 121737
• NCBI Official Full Name: rab GTPase-activating protein 1
• NCBI Official Synonym Full Names: RAB GTPase activating protein 1
• NCBI Official Symbol: RABGAP1
• NCBI Official Synonym Symbols: GAPCENA; TBC1D11
• NCBI Protein Information: rab GTPase-activating protein 1
• UniProt Protein Name: Rab GTPase-activating protein 1
• UniProt Gene Name: RABGAP1
• UniProt Entry Name: RBGP1_HUMAN