IP (Immunoprecipitation)
(Detection of human RABGEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody AAA212071 (lot AAA212071-2) used for IP at 3 ug per reaction. RABGEF1 was also immunoprecipitated by rabbit anti-RABGEF1 antibody For blotting immunoprecipitated RABGEF1, was used at 1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human RABGEF1 Polyclonal Antibody | anti-RABGEF1 antibody
Rabbit anti-RABGEF1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
RABGEF1, Antibody; Rabbit anti-RABGEF1 Antibody, Affinity Purified; RAB guanine nucleotide exchange factor (GEF) 1; rab5 GDP/GTP exchange factor; rabaptin-5-associated exchange factor for Rab5; RABEX5; rabex-5; RAP1; Rab5 GDP/GTP exchange factor; RAB guanine nucleotide exchange factor 1; anti-RABGEF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RABGEF1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse, Bovine
Immunogen
Between 275 and 325
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human RABGEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody AAA212071 (lot AAA212071-2) used for IP at 3 ug per reaction. RABGEF1 was also immunoprecipitated by rabbit anti-RABGEF1 antibody For blotting immunoprecipitated RABGEF1, was used at 1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human RABGEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody AAA212071 (lot AAA212071-2) used for IP at 3 ug per reaction. RABGEF1 was also immunoprecipitated by rabbit anti-RABGEF1 antibody For blotting immunoprecipitated RABGEF1, was used at 1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human RABGEF1 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-RABGEF1 (Cat. No. AAA212071 Lot2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human RABGEF1 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-RABGEF1 (Cat. No. AAA212071 Lot2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-RABGEF1 antibody
RabGEF1 (Rab guanine nucleotide exchange factor 1) is a rab effector protein acting as linker between gamma-adaptin, Rab4A or Rab5A. RabGEF1 is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It stimulates nucleotide exchange on Rab5A. RabGEF1 can act as a ubiquitin ligase [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UJ41].
Product Categories/Family for anti-RABGEF1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The RABGEF1 rabgef1 (Catalog #AAA212071) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RABGEF1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RABGEF1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the RABGEF1 rabgef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RABGEF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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