Rabbit RAD9 Polyclonal Antibody | anti-RAD9A antibody

RAD9 Antibody (BH3 Domain Specific)

Cat. Num. AAA286319

• Gene Names: RAD9A; RAD9
• Reactivity: Human, mouse (Predicted Reactivity: Monkey)
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RAD9, Antibody; RAD9 Antibody (BH3 Domain Specific); Cell cycle checkpoint control protein RAD9A; hRAD9; DNA repair exonuclease rad9 homolog A; RAD9A; anti-RAD9A antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Monkey)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human RAD9.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 1.8 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 315

• Applicable Applications for anti-RAD9A antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Western blot analysis of Rad9(arrow) using rabbit polyclonal Rad9 BH3 domain Pab. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Rad9 gene (Lane 2) (Origene Technologies).)

WB (Western Blot)

(Western blot analysis of anti-Rad9 BH3 domain Pab in HL-60 cell lysate. Rad9 BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Related Product Information for anti-RAD9A antibody

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.

References

Hopkins, K.M., et al., Cancer Res. 63(17):5291-5298 (2003).
Greer, D.A., et al., Cancer Res. 63(16):4829-4835 (2003).
St Onge, R.P., et al., J. Biol. Chem. 278(29):26620-26628 (2003).
Roos-Mattjus, P., et al., J. Biol. Chem. 278(27):24428-24437 (2003).
Yoshida, K., et al., EMBO J. 22(6):1431-1441 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 342672046
• NCBI GeneID: 5883
• NCBI Accession #: NP_001230153.1; NP_004575.1
• NCBI GenBank Nucleotide #: NM_001243224.1
• UniProt Accession #: Q99638; Q6FI29; Q96C41; B2RCZ8
• Molecular Weight: 42547
• NCBI Official Full Name: cell cycle checkpoint control protein RAD9A isoform 2
• NCBI Official Synonym Full Names: RAD9 checkpoint clamp component A
• NCBI Official Symbol: RAD9A
• NCBI Official Synonym Symbols: RAD9
• NCBI Protein Information: cell cycle checkpoint control protein RAD9A
• UniProt Protein Name: Cell cycle checkpoint control protein RAD9A
• UniProt Gene Name: RAD9A
• UniProt Synonym Gene Names: hRAD9
• UniProt Entry Name: RAD9A_HUMAN